ELISA/assay

Human Neurogenic locus notch homolog protein 4, NOTCH4 ELISA Kit

MBS900186

48-Strip-Wells

510 USD

ELISA Kit

Human Neurogenic locus notch homolog protein 4, NOTCH4 ELISA Kit

Notch homolog 4 (Drosophila)

Human Neurogenic locus notch homolog protein 4 (NOTCH4) ELISA kit; DAQB-143M3.4; FLJ16302; INT3; MGC74442; NOTCH3; notch4; Notch homolog 4 (Drosophila)

neurogenic locus notch homolog protein 4 preproprotein; Neurogenic locus notch homolog protein 4; neurogenic locus notch homolog protein 4; Notch homolog 4; notch 4

NOTCH4

NOTCH4; NOTCH4; INT3; INT3; Notch 4; hNotch4

NOTC4_HUMAN

Human

2003

This assay has high sensitivity and excellent specificity for detection of Human NOTCH4. No significant cross-reactivity or interference between Human NOTCH4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOTCH4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOTCH4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOTCH4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOTCH4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

55770876

NP_004548.3

NM_004557.3

Q99466

209,622 Da

Delta-Notch Signaling Pathway (198879); Dorso-ventral Axis Formation Pathway (114227); Dorso-ventral Axis Formation Pathway (472); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Neural Crest Differentiation Pathway (672460); Notch Signaling Pathway (198891); Notch Signaling Pathway (83062)

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. This gene may be associated with susceptibility to schizophrenia in a small portion of cases. An alternative splice variant has been described but its biological nature has not been determined. [provided by RefSeq, Jul 2008]

NOTCH4: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4. Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. Belongs to the NOTCH family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cell surface; integral to plasma membrane; plasma membrane; extracellular region; nucleus; cytosol. Molecular Function: protein binding; protein heterodimerization activity; receptor activity; calcium ion binding. Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; positive regulation of transcription, DNA-dependent; Notch receptor processing; morphogenesis of a branching structure; cell fate determination; activation of Notch receptor target transcription factor; patterning of blood vessels; embryonic development; negative regulation of cell differentiation; negative regulation of endothelial cell differentiation; mammary gland development; gene expression; hemopoiesis; endothelial cell morphogenesis; cell differentiation

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800