catalog number :
MBS900184
products type :
ELISA Kit
products full name :
Human Lymphocyte antigen 86, LY86 ELISA Kit
products short name :
lymphocyte antigen 86
products name syn :
Human Lymphocyte antigen 86 (LY86) ELISA kit; RP1-80N2.1; MD-1; MMD-1; dJ80N2.1; MD-1; RP105-associated; OTTHUMP00000015993; lymphocyte antigen 86
other names :
lymphocyte antigen 86; Lymphocyte antigen 86; lymphocyte antigen 86; ly-86; protein MD-1; MD-1, RP105-associated; lymphocyte antigen 86; Protein MD-1
products gene name :
LY86
other gene names :
LY86; LY86; MD-1; MMD-1; dJ80N2.1; MD1; Ly-86
uniprot entry name :
LY86_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human LY86. No significant cross-reactivity or interference between human LY86 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LY86 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LY86 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LY86 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LY86 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_004262.1
ncbi gb acc num :
NM_004271.3
ncbi mol weight :
17,906 Da
ncbi pathways :
Immune System Pathway (106386); Innate Immune System Pathway (106387); Toll Like Receptor 4 (TLR4) Cascade Pathway (106398); Toll-Like Receptors Cascades Pathway (106388)
uniprot summary :
LY86: May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression. Protein type: Apoptosis; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6p25.1. Cellular Component: extracellular space; plasma membrane. Biological Process: cell proliferation; apoptosis; positive regulation of lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; humoral immune response
size4 :
10x96-Strip-Wells