ELISA/assay

Human SH3 and multiple ankyrin repeat domains protein 3, SHANK3 ELISA Kit

MBS900106

48-Strip-Wells

510 USD

ELISA Kit

Human SH3 and multiple ankyrin repeat domains protein 3, SHANK3 ELISA Kit

SH3 and multiple ankyrin repeat domains 3

Human SH3 and multiple ankyrin repeat domains protein 3 (SHANK3) ELISA kit; DEL22q13.3; KIAA1650; PROSAP2; PSAP2; SPANK-2; proline rich synapse associated protein 2; proline-rich synapse-associated protein 2; shank postsynaptic density protein; SH3 and multiple ankyrin repeat domains 3

SH3 and multiple ankyrin repeat domains protein 3; SH3 and multiple ankyrin repeat domains protein 3; SH3 and multiple ankyrin repeat domains protein 3; shank postsynaptic density protein; proline rich synapse associated protein 2; SH3 and multiple ankyrin repeat domains 3; Proline-rich synapse-associated protein 2; ProSAP2

SHANK3

SHANK3; SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3; KIAA1650; PROSAP2; PSAP2; Shank3; ProSAP2

SHAN3_HUMAN

Human

1731

This assay has high sensitivity and excellent specificity for detection of human SHANK3. No significant cross-reactivity or interference between human SHANK3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SHANK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SHANK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SHANK3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SHANK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

380748963

NP_277052.1

NM_033517.1

Q9BYB0

184,667 Da

Glutamatergic Synapse Pathway (213818); Glutamatergic Synapse Pathway (213811); Signaling Events Regulated By Ret Tyrosine Kinase Pathway (137915)

This gene is a member of the Shank gene family. Shank proteins are multidomain scaffold proteins of the postsynaptic density that connect neurotransmitter receptors, ion channels, and other membrane proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Shank proteins also play a role in synapse formation and dendritic spine maturation. Mutations in this gene are a cause of autism spectrum disorder (ASD), which is characterized by impairments in social interaction and communication, and restricted behavioral patterns and interests. Mutations in this gene also cause schizophrenia type 15, and are a major causative factor in the neurological symptoms of 22q13.3 deletion syndrome, which is also known as Phelan-McDermid syndrome. Additional isoforms have been described for this gene but they have not yet been experimentally verified. [provided by RefSeq, Mar 2012]

SHANK3: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B. Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome). Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15). SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 22q13.3. Cellular Component: postsynaptic membrane; neuron projection; cytoplasm; dendritic spine; plasma membrane; cell junction. Molecular Function: protein C-terminus binding; ionotropic glutamate receptor binding; identical protein binding; protein binding; zinc ion binding; GKAP/Homer scaffold activity; actin binding; SH3 domain binding. Biological Process: positive regulation of synapse structural plasticity; negative regulation of actin filament bundle formation; synaptogenesis; striatal medium spiny neuron differentiation; adult behavior; MAPKKK cascade; brain morphogenesis; vocal learning; learning; social behavior; positive regulation of synaptic transmission, glutamatergic; positive regulation of long-term neuronal synaptic plasticity; negative regulation of cell volume; memory. Disease: Schizophrenia 15; Phelan-mcdermid Syndrome

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800