ARNT Antibody | MyBioSource MBS859631 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ARNT Antibody

catalog :

MBS859631

quantity :

0.1 mg

price :

290 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS859631

products type :

Antibody

products full name :

ARNT Antibody

products short name :

ARNT

other names :

Homo sapiens aryl hydrocarbon receptor nuclear translocator, mRNA; Aryl hydrocarbon receptor nuclear translocator; aryl hydrocarbon receptor nuclear translocator; dioxin receptor, nuclear translocator; class E basic helix-loop-helix protein 2; hypoxia-inducible factor 1, beta subunit; aryl hydrocarbon receptor nuclear translocator; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta

products gene name :

ARNT

other gene names :

ARNT; ARNT; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta; BHLHE2; ARNT protein; bHLHe2; HIF-1-beta; HIF1-beta

uniprot entry name :

ARNT_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human

sequence length :

3720

form :

Lyophilized ARNT antibody purified by protein affinity column containing 1% BSA and 0.02% NaN3

concentration :

100ug/200ul

tested application :

ELISA (EIA), Western Blot (WB)

app notes :

ELISA titer: 1:20,000-1:80,000. Western blot analysis: 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the end user.

other info1 :

Source Note: Rabbit polyclonal ARNT (1) antibody was raised against a recombinate human ARNT protein 125-296aa (BC060838). Reactivity Note: Predicted: Mouse, Rat; Not yet tested in other species.

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

(1) Wang,Y., Li,Y., Wang,D., et al. Suppression of the hypoxia inducible factor-1 function by redistributing the aryl hydrocarbon receptor nuclear translocator from nucleus to cytoplasm. Cancer Lett. 320 (1), 111-121 (2012)

ncbi gi num :

38173805

ncbi acc num :

BC060838

ncbi mol weight :

86,366 Da

ncbi pathways :

AhR Pathway (755436); Angiogenesis Pathway (198772); Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); HIF-2-alpha Transcription Factor Network Pathway (137956); Hypoxic And Oxygen Homeostasis Regulation Of HIF-1-alpha Pathway (138056)

ncbi summary :

This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]

uniprot summary :

ARNT: a bHLH transcription factor that requires dimerization with another bHLH protein for efficient DNA binding. A binding partner for the Ah (dioxin) nuclear receptor. Required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. A binding partner for HIF1A or HIF2A, transcription factors that regulates transcription from RNA polymerase II promoter in the adaptive response to hypoxia and oxidative stress. This complex activates target genes that limit oxygen consumption. HIF-1alpha may have nontranscriptional activities that inhibit DNA replication and cell proliferation when oxygen becomes scarce. Forms heterodimers with other bHLH proteins. Interacts with TACC3. Three isoforms of the human protein are produced by alternative splicing. Protein type: Nuclear receptor; Transcription factor; Motility/polarity/chemotaxis; DNA-binding. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; aryl hydrocarbon receptor binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; aryl hydrocarbon receptor activity; transcription factor binding; transcription factor activity. Biological Process: embryonic placenta development; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; mRNA transcription from RNA polymerase II promoter; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of protein sumoylation; positive regulation of hormone biosynthetic process; positive regulation of glycolysis; response to hypoxia; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; cell differentiation

size1 :

0.1 mg

price1 :

290 USD

more info or order :

MyBioSource product webpage