antibody

Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody

MBS859605

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody

Retinoic Acid Receptor alpha (Phospho-Ser77)

NR1B1; RAR-alpha; Retinoic acid receptor alpha; retinoic acid receptor, alpha; RRA

retinoic acid receptor alpha isoform 1; Retinoic acid receptor alpha; retinoic acid receptor alpha; RAR-alpha; retinoic acid receptor, alpha polypeptide; nuclear receptor subfamily 1 group B member 1; retinoic acid nuclear receptor alpha variant 1; retinoic acid nuclear receptor alpha variant 2; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form; retinoic acid receptor, alpha; Nuclear receptor subfamily 1 group B member 1

RARA; RARA; RAR; NR1B1; NR1B1; RAR-alpha

RARA_HUMAN

Rabbit

Human, Mouse, Rat

462

Retinoic Acid Receptor alpha (Phospho-Ser77) antibody detects endogenous levels of Retinoic Acid Receptor alpha only when phosphorylated at serine 77.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation s

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C/1 year

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

All Sites: Human: Ser77; Mouse: Ser77; Rat: Ser74

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Retinoic Acid Receptor alpha around the phosphorylation site of serine 77 (P-P-SP-P-P). Reactivity Notes: Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Giguere V., Nature 330:624-629(1987). Chen Z., Proc. Natl. Acad. Sci. U.S.A. 91:1178-1182(1994). Hjalt T.A.H., Mamm. Genome 10:528-529(1999).

4506419

NP_000955.1

NM_000964.3

P10276

39,700 Da

Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adipogenesis Pathway (198832); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); IL-3 Signaling Pathway (198881); Integrated Pancreatic Cancer Pathway (711360); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198887)

This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010]

RARA: is a receptor for retinoic acid, a potent mammalian morphogen and teratogen that has profound effects on vertebrate development. RARA is a member of the nuclear receptor superfamily. Controls cell function by directly regulating gene expression. Its phosphorylation is crucial for transcriptional activity. Aberrations involving RARA may be a cause of acute promyelocytic leukemia. Two splice-variant isoforms have been described. Protein type: Nuclear receptor; DNA-binding; Transcription factor; Oncoprotein. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: nucleoplasm; cell surface; cell soma; perinuclear region of cytoplasm; cytoplasm; nuclear chromatin; dendrite; nucleus; actin cytoskeleton. Molecular Function: protein domain specific binding; protein kinase B binding; retinoic acid binding; zinc ion binding; chromatin DNA binding; transcription coactivator activity; translation repressor activity, nucleic acid binding; phosphoinositide 3-kinase regulator activity; drug binding; alpha-actinin binding; transcription factor binding; protein binding; enzyme binding; protein heterodimerization activity; protein kinase A binding; steroid hormone receptor activity; mRNA 5 -UTR binding; retinoic acid receptor activity; transcription factor activity; transcription corepressor activity; receptor binding. Biological Process: limb development; retinoic acid receptor signaling pathway; prostate gland development; negative regulation of translational initiation; regulation of myelination; estrogen receptor signaling pathway; glandular epithelial cell development; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell differentiation; regulation of synaptic plasticity; female pregnancy; negative regulation of transcription from RNA polymerase II promoter; signal transduction; protein amino acid phosphorylation; regulation of phosphoinositide 3-kinase activity; response to estradiol stimulus; response to vitamin A; germ cell development; negative regulation of granulocyte differentiation; positive regulation of interleukin-4 production; negative regulation of cell proliferation; Sertoli cell fate commitment; positive regulation of T-helper 2 cell differentiation; ureteric bud development; negative regulation of interferon-gamma production; positive regulation of cell proliferation; positive regulation of interleukin-13 production; transmembrane transport; positive regulation of interleukin-5 production; transcription initiation from RNA polymerase II promoter; response to retinoic acid; multicellular organism growth; positive regulation of cell cycle; positive regulation of binding; negative regulation of tumor necrosis factor production; liver development; embryonic camera-type eye development; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; response to ethanol; response to cytokine stimulus; neural tube closure; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent; apoptotic cell clearance; negative regulation of apoptosis. Disease: Acute Promyelocytic Leukemia

0.1 mg

215 USD