CD4 (Phospho-Ser433) Antibody | MyBioSource MBS859599 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD4 (Phospho-Ser433) Antibody

catalog :

MBS859599

quantity :

0.1 mg

price :

280 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

P-Peptide: - / + . Immunohistochemistry analysis of paraffin-embedded human brain tissue using CD4 (Phospho-Ser433) antibody.

image 2 :

P-Peptide: - / + . Immunofluorescence analysis of HepG2 cells, using CD4 (Phospho-Ser433) antibody.

product information

catalog number :

MBS859599

products type :

Antibody

products full name :

CD4 (Phospho-Ser433) Antibody

products short name :

[CD4]

products name syn :

[CD4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 precursor]

other names :

[T-cell surface glycoprotein CD4 isoform 1; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 molecule; T-cell surface antigen T4/Leu-3; CD_antigen: CD4]

products gene name :

[CD4]

other gene names :

[CD4; CD4; CD4mut]

clonality :

polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Human

sequence length :

458

specificity :

CD4 (Phospho-Ser433) antibody detects endogenous levels of CD4 only when phosphorylated at serine 433.

purity :

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

form :

In phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at-20 degree C for 1 year.

tested application :

Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

app notes :

IHC: 1:50~1:100. IF: 1:100~1:500. ELISA: 1:40000

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human CD4 around the phosphorylation site of serine 433 (R-M-S P -Q-I).

other info2 :

All Sites: Human: Ser433

products categories :

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

products references :

GR Harriman, J. Immunol., Oct 1990; 145: 2406. G Zauli, Blood, Sep 1994; 84: 1896-1905. Andrew Zloza, Blood, Sep 2003; 102: 2156-2164. David M. Rothstein, Int. Immunol., Jan 1993; 5: 409-418.

ncbi gi num :

10835167

ncbi acc num :

NP_000607.1

ncbi gb acc num :

NM_000616.4

uniprot acc num :

P01730

ncbi pathways :

Adaptive Immune System Pathway (366160); Alpha-defensins Pathway (530758); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Arf1 Pathway (137927); Binding And Entry Of HIV Virion Pathway (105986); C-MYB Transcription Factor Network Pathway (138073); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

ncbi summary :

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

uniprot summary :

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

size1 :

0.1 mg

price1 :

280 USD

more info or order :

MyBioSource product webpage