A-RAF (Phospho-Tyr302) Antibody | MyBioSource MBS859189 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

A-RAF (Phospho-Tyr302) Antibody

catalog :

MBS859189

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS859189

products type :

Antibody

products full name :

A-RAF (Phospho-Tyr302) Antibody

products short name :

A-RAF

products name syn :

A-RAF; A-Raf proto-oncogene serine/threonine-protein kinase; A-raf-1; ARAF; ARAF1; EC 2.7.11.1; PKS; PKS2; Proto-oncogene Pks; Raf; kinase A-Raf

other names :

serine/threonine-protein kinase A-Raf isoform 2; Serine/threonine-protein kinase A-Raf; serine/threonine-protein kinase A-Raf; Oncogene ARAF1; proto-oncogene Pks; proto-oncogene A-Raf-1; Ras-binding protein DA-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; A-Raf proto-oncogene serine/threonine-protein kinase; A-Raf proto-oncogene, serine/threonine kinase; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks

products gene name :

A-RAF

other gene names :

ARAF; ARAF; PKS2; A-RAF; ARAF1; RAFA1; ARAF1; PKS; PKS2

uniprot entry name :

ARAF_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

609

specificity :

A-RAF (Phospho-Tyr301/302) antibody detects endogenous levels of A-RAF only when phosphorylated at tyrosine 301/tyrosine 302.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation s

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

app notes :

WB: 1:500~1:1000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:20000

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human A-RAF around the phosphorylation site of tyrosine 301/tyrosine 302 (G-Y-YP-W-E). All Sites: Human: Tyr301/Tyr302; Mouse: Tyr299/Tyr300; Rat: Tyr299/Tyr300

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Oliver G. Rössler. Am J Physiol Cell Physiol, May 2004; 286: C1118 - C1129. Marie A. Bogoyevitch. J. Biol. Chem., Nov 1995; 270: 26303. Weicheng Liu. J. Biol. Chem., Mar 2004; 279: 10167 - 10175. M Huleihel. Mol. Cell. Biol., Jul 1986; 6: 2655 - 2662.

ncbi gi num :

371875793

ncbi acc num :

NP_001243125.1

ncbi gb acc num :

NM_001256196.1

uniprot acc num :

P10398

ncbi mol weight :

20,904 Da

ncbi pathways :

Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Chronic Myeloid Leukemia Pathway (83116); Chronic Myeloid Leukemia Pathway (528); Colorectal Cancer Pathway (83106); Colorectal Cancer Pathway (518)

ncbi summary :

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]

uniprot summary :

ARAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib. Protein type: Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, TKL; EC 2.7.11.1; TKL group; RAF family. Chromosomal Location of Human Ortholog: Xp11.4-p11.2. Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; receptor signaling protein activity; ATP binding; protein kinase activity. Biological Process: regulation of proteasomal ubiquitin-dependent protein catabolic process; protein modification process; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; negative regulation of apoptosis; regulation of TOR signaling pathway

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage