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SAPK/JNK (Phospho-Tyr185) Antibody

MBS859168

0.1 mg

280 USD

Antibody

SAPK/JNK (Phospho-Tyr185) Antibody

[SAPK/JNK]

[c-Jun N-terminal kinase 1; EC 2.7.11.24; JNK-46; JNK1-alpha-2; kinase JNK1; MAPK8; Mitogen-activated protein kinase 8; MK08; p54 gamma; PRKM8; SAPK gamma; Stress-activated protein kinase JNK1]

[mitogen-activated protein kinase 8 isoform beta2; Mitogen-activated protein kinase 8; mitogen-activated protein kinase 8; mitogen-activated protein kinase 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1]

[SAPK/JNK]

[MAPK8; MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c]

Human, Mouse, Rat

427

SAPK/JNK (Phospho-Tyr185) antibody detects endogenous levels of SAPK/JNK only when phosphorylated at tyrosine 185.

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at-20 degree C for 1 year.

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

WB: 1:500~1:1000. IHC: 1:50~1:100. ELISA: 1:20000

Testing Data

Immunohistochemistry (IHC)

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human SAPK/JNK around the phosphorylation site of tyrosine 185 (T-P-YP-V-V).

All Sites: Human: Tyr185. Mouse: Tyr185. Rat: Tyr185

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

Derijard B., Cell 76:1025-1037(1994). Gupta S., EMBO J. 15:2760-2770(1996). Derijard B., Science 267:682-685(1995).

513788279

NP_001265476.1

NM_001278547.1

P45983

35,333 Da

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Activated TLR4 Signalling Pathway (106400); Activation Of BH3-only Proteins Pathway (105658); Activation Of BIM And Translocation To Mitochondria Pathway (105662); Activation Of BMF And Translocation To Mitochondria Pathway (105663); Activation Of The AP-1 Family Of Transcription Factors Pathway (160139); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917)

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973).

0.1 mg

280 USD