CD18/ITGB2 (Phospho-Thr758) Antibody | MyBioSource MBS858983 product information

product summary

company name :

MyBioSource

product type :

other

product name :

CD18/ITGB2 (Phospho-Thr758) Antibody

catalog :

MBS858983

quantity :

0.1 mg

price :

280 USD

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from COS cells, treated with EGF (200ng/ml, 30mins), using CD18/ITGB2 (Phospho-Thr758) antibody.

image 2 :

Immunofluorescence analysis of HepG2 cells, using CD18/ITGB2 (Phospho-Thr758) antibody.

product information

catalog number :

MBS858983

products type :

Antibody

products full name :

CD18/ITGB2 (Phospho-Thr758) Antibody

products short name :

[CD18/ITGB2]

products name syn :

[CD18; cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 beta; complement receptor C3 beta; integrin beta-2; ITB2]

other names :

[integrin beta-2 isoform 1; Integrin beta-2; integrin beta-2; integrin subunit beta 2; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD_antigen: CD18]

products gene name :

[CD18/ITGB2]

other gene names :

[ITGB2; ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1; CD18; MFI7]

reactivity :

Human, Mouse, Rat

sequence length :

769

specificity :

CD18/ITGB2 (Phospho-Thr758) antibody detects endogenous levels of CD18/ITGB2 only when phosphorylated at threonine 578.

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at-20 degree C for 1 year.

tested application :

Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)

app notes :

WB: 1:500~1:1000. IF: 1:100~1:500. ELISA: 1:40000

image1 heading :

Western blot

image2 heading :

Immunofluorescence

other info1 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human CD18/ITGB2 around the phosphorylation site of threonine 758 (S-A-TP-T-T).

other info2 :

All Sites: Human: Thr758. Mouse: Thr760. Rat: Thr757. Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

products categories :

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

products references :

Kishimoto T.K., Cell 48:681-690(1987). Weitzman J.B., FEBS Lett. 294-97-103(1991). Hattori M., Nature 405:311-319(2000).

ncbi gi num :

735367775

ncbi acc num :

NP_000202.3

ncbi gb acc num :

NM_000211.4

uniprot acc num :

P05107

ncbi mol weight :

84,782 Da

ncbi pathways :

Adaptive Immune System Pathway (1269171); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); CXCR3-mediated Signaling Events Pathway (138011); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Extracellular Matrix Organization Pathway (1270244)

ncbi summary :

This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

uniprot summary :

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992, PubMed:28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407).

size1 :

0.1 mg

price1 :

280 USD

more info or order :

MyBioSource product webpage