ADAM 17 (Phospho-Thr735) Antibody | MyBioSource MBS858804 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ADAM 17 (Phospho-Thr735) Antibody

catalog :

MBS858804

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS858804

products type :

Antibody

products full name :

ADAM 17 (Phospho-Thr735) Antibody

products short name :

ADAM 17

products name syn :

ADA17; ADAM 17; CD156b antigen; CSVP; EC 3.4.24.86; TACE; TNF-alpha convertase; TNF-alpha-converting enzyme; a disintegrin and metalloproteinase domain 17; snake venom-like protease

other names :

disintegrin and metalloproteinase domain-containing protein 17 preproprotein; Disintegrin and metalloproteinase domain-containing protein 17; disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; snake venom-like protease; TNF-alpha converting enzyme; ADAM metallopeptidase domain 18; tumor necrosis factor, alpha, converting enzyme; ADAM metallopeptidase domain 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD_antigen: CD156b

products gene name :

ADAM 17

other gene names :

ADAM17; ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1; CSVP; TACE; ADAM 17

uniprot entry name :

ADA17_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

824

specificity :

ADAM 17 (Phospho-Thr735) antibody detects endogenous levels of ADAM 17 only when phosphorylated at threonine 735.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB: 1:500~1:1000, ELISA: 1:20000

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ADAM 17 around the phosphorylation site of threonine 735 (P-Q-TP-P-G). All Sites: Human: Thr735; Mouse: Thr735; Rat: Thr735

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Moss M.L., Nature 385:733-736(1997). Patel I.R., J. Immunol. 160:4570-4579(1998). Diaz-Rodriguez E., Mol. Biol. Cell 13:2031-2044(2002). Olsen J.V., Cell 127:635-648(2006).

ncbi gi num :

73747889

ncbi acc num :

NP_003174.3

ncbi gb acc num :

NM_003183.4

uniprot acc num :

P78536

ncbi mol weight :

78,543 Da

ncbi pathways :

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (576270); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Apoptosis Pathway (105648); BDNF Signaling Pathway (712093); Collagen Degradation Pathway (730309); Constitutive Signaling By NOTCH1 HD Domain Mutants Pathway (771591); Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway (771595); Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway (771593)

ncbi summary :

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

uniprot summary :

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala- -Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.4.24.86; Protease. Chromosomal Location of Human Ortholog: 2p25. Cellular Component: cell surface; focal adhesion; membrane; integral to plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; intercellular junction; actin cytoskeleton; lipid raft. Molecular Function: integrin binding; protein binding; interleukin-6 receptor binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; Notch binding; SH3 domain binding; PDZ domain binding. Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; cell motility involved in cell locomotion; T cell differentiation in the thymus; neutrophil mediated immunity; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; extracellular matrix disassembly; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; germinal center formation; negative regulation of interleukin-8 production; cell adhesion; response to drug; epidermal growth factor receptor signaling pathway; spleen development; Notch signaling pathway; response to high density lipoprotein stimulus; membrane protein intracellular domain proteolysis; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; collagen catabolic process; regulation of mast cell apoptosis; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; wound healing, spreading of epidermal cells. Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage