Smad3 (Phospho-Thr179) Antibody | MyBioSource MBS858478 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Smad3 (Phospho-Thr179) Antibody

catalog :

MBS858478

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS858478

products type :

Antibody

products full name :

Smad3 (Phospho-Thr179) Antibody

products short name :

Smad3

products name syn :

JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3

other names :

mothers against decapentaplegic homolog 3 isoform 2; Mothers against decapentaplegic homolog 3; mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3; SMAD family member 3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

products gene name :

Smad3

other gene names :

SMAD3; SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3

uniprot entry name :

SMAD3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

320

specificity :

Smad3 (Phospho-Thr179) antibody detects endogenous levels of Smad3 only when phosphorylated at threonine 179.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

app notes :

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:1000

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of threonine 179 (P-E-TP-P-P). All Sites: Human: Thr179; Mouse: Thr179; Rat: Thr179

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Zhang Y., Nature 383:168-172(1996). Riggins G.J., Nat. Genet. 13:347-349(1996). Arai T., Cancer Lett. 122:157-163(1998).

ncbi gi num :

223029440

ncbi acc num :

NP_001138574.1

ncbi gb acc num :

NM_001145102.1

uniprot acc num :

P84022

ncbi mol weight :

25,722 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Adipogenesis Pathway (198832); Alpha6-Beta4 Integrin Signaling Pathway (198807); Androgen Receptor Signaling Pathway (198806); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147809)

ncbi summary :

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

uniprot summary :

SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton. Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 15q22.33. Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; plasma membrane; nuclear inner membrane; cytosol; nucleus; receptor complex. Molecular Function: identical protein binding; ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; beta-catenin binding; protein kinase binding; transcription factor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; phosphatase binding; collagen binding; protein binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor activity; transforming growth factor beta receptor binding. Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; embryonic foregut morphogenesis; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; regulation of transcription from RNA polymerase II promoter; positive regulation of chondrocyte differentiation; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; negative regulation of apoptosis; endoderm development; evasion of host defenses by virus; T cell activation; wound healing; primary microRNA processing; negative regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of focal adhesion formation; transport; ureteric bud development; positive regulation of transforming growth factor-beta3 production; positive regulation of transcription factor import into nucleus; thyroid gland development; heart looping; caspase activation; transcription initiation from RNA polymerase II promoter; intercellular junction assembly and maintenance; regulation of transforming growth factor-beta2 production; regulation of immune response; regulation of binding; protein stabilization; in utero embryonic development; positive regulation of bone mineralization; liver development; SMAD protein complex assembly; immune system development; positive regulation of interleukin-1 beta production; negative regulation of inflammatory response; response to hypoxia; immune response; gene expression; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration. Disease: Loeys-dietz Syndrome 3

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage