ATP-Citrate Lyase (ACLY) Antibody | MyBioSource MBS857833 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ATP-Citrate Lyase (ACLY) Antibody

catalog :

MBS857833

quantity :

0.1 mg

price :

300 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Immunocytochemistry of HeLa cells using anti- ATP-Citrate Lyase (C-terminus) antibody diluted 1:150.

image 2 :

Western blot detection of ATP-Citrate Lyase antibody in K562&Hela cell lysates using ATP-Citrate Lyase antibody (1:1000 diluted). Predicted band size: 120KDa Observed band size: 120KDa.

image 3 :

Flow Cytometry analysis of HeLa cells stained with ATP-Citrate Lyase (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.

product information

catalog number :

MBS857833

products type :

Antibody

products full name :

ATP-Citrate Lyase (ACLY) Antibody

products short name :

[ATP-Citrate Lyase]

products name syn :

[ACL; ATPCL; CLATP]

other names :

[ATP-citrate synthase isoform 1; ATP-citrate synthase; ATP-citrate synthase; ATP citrate lyase; ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme]

products gene name :

[ACLY]

other gene names :

[ACLY; ACLY; ACL; ATPCL; CLATP; ACL]

clonality :

Monoclonal

isotype :

IgG2a

host :

Mouse

reactivity :

Human

sequence length :

1101

specificity :

This antibody detects endogenous levels ATP-Citrate Lyase and does not cross-react with related proteins.

purity :

Affinity purified

form :

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol

tested application :

Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

image1 heading :

Testing Data

image2 heading :

Testing Data #2

image3 heading :

Testing Data #3

other info1 :

Immunogen: Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E Coli. Cellular Localization: Cytoplasm

products categories :

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

products description :

UniPort Summary Function: ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Entrez Summary: ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

ncbi gi num :

38569421

ncbi acc num :

NP_001087.2

ncbi gb acc num :

NM_001096

uniprot acc num :

P53396

ncbi mol weight :

120kDa

ncbi pathways :

ChREBP Activates Metabolic Gene Expression Pathway (106104); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Fatty Acid Biosynthesis Pathway (198873); Fatty Acyl-CoA Biosynthesis Pathway (106115); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Integration Of Energy Metabolism Pathway (106096); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); SREBP Signalling Pathway (219802)

ncbi summary :

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

uniprot summary :

ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage