HSF1 (Phospho-Ser307) Antibody | MyBioSource MBS857430 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HSF1 (Phospho-Ser307) Antibody

catalog :

MBS857430

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS857430

products type :

Antibody

products full name :

HSF1 (Phospho-Ser307) Antibody

products short name :

HSF1

other names :

heat shock factor protein 1; Heat shock factor protein 1; heat shock factor protein 1; HSF 1; HSTF 1; heat shock transcription factor 1; Heat shock transcription factor 1; HSTF 1

products gene name :

HSF1

other gene names :

HSF1; HSF1; HSTF1; HSTF1; HSF 1; HSTF 1

uniprot entry name :

HSF1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

529

specificity :

HSF1 (Phospho-Ser307) Antibody detects endogenous levels of HSF1 only when phosphorylated at serine 307.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

50ug/50ul

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

WB: 1:500~1:1000, IF: 1:100-200

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-SP-P-R). Pathway: MAPK, Chromatin/Transcription

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4. Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026. Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.

ncbi gi num :

5031767

ncbi acc num :

NP_005517.1

ncbi gb acc num :

NM_005526.2

uniprot acc num :

Q00613

ncbi mol weight :

52,881 Da

ncbi pathways :

Attenuation Phase Pathway (980473); Cellular Response To Heat Stress Pathway (980470); Cellular Responses To Stress Pathway (645258); HSF1 Activation Pathway (980471); HSF1-dependent Transactivation Pathway (980472); Legionellosis Pathway (469200); Legionellosis Pathway (469186); Regulation Of HSF1-mediated Heat Shock Response Pathway (980474)

ncbi summary :

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: nucleoplasm; protein complex; pronucleus; cytoplasm; cytosol. Molecular Function: protein binding; chromatin binding; transcription factor activity. Biological Process: embryonic placenta development; embryonic process involved in female pregnancy; female meiosis; defense response; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; positive regulation of multicellular organism growth; protein amino acid phosphorylation; negative regulation of cell proliferation; mRNA transcription; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis

size1 :

0.1 mg

price1 :

215 USD

size2 :

0.05 mg

price2 :

225

more info or order :

MyBioSource product webpage