antibody

Myosin Light Chain 2 (Phospho-Ser18) Antibody

MBS857334

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

Antibody

Myosin Light Chain 2 (Phospho-Ser18) Antibody

Myosin Light Chain 2

DNA-dependent protein kinase catalytic subunit isoform 2; DNA-dependent protein kinase catalytic subunit; DNA-dependent protein kinase catalytic subunit; p460; DNA-PK catalytic subunit; hyper-radiosensitivity of murine scid mutation, complementing 1; protein kinase, DNA-activated, catalytic polypeptide; DNPK1; p460

PRKDC; PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs; HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs

PRKDC_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

4097

Myosin Light Chain 2 (Phospho-Ser18) Antibody detects endogenous levels of Myosin Light Chain 2 only when phosphorylated at serine 18.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

50ug/50ul

Store at -20 degree C for 1 year

Immunohistochemistry (IHC)

WB: 1:500~1:1000, IHC: 1:50~1:100

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Myosin Light Chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V). Pathway: Cytoskeletal/Adhesion

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619. Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627. Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256

126032350

NP_001075109.1

NM_001081640.1

P78527

465,501 Da

BARD1 Signaling Events Pathway (137959); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Coregulation Of Androgen Receptor Activity Pathway (138085); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DNA Repair Pathway (105837); DNA-PK Complex Pathway (413430); DNA-PK Complex Pathway (890556)

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

DNAPK: an atypical protein kinase of the PIKK family. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. and modulation of transcription. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Defects cause severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections. Required for repair of radiation-induced dsDNA breaks. Loss of function in mice or horses leads to the SCID (severe combined immune deficiency) phenotype due to failure of immunoglobulin rearrangement. Target of mutation in mismatch repair-deficient colorectal cancer. Inhibitor: KU-7059. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA repair, damage; EC 2.7.11.1; Nucleolus; Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; DNAPK subfamily. Chromosomal Location of Human Ortholog: 8q11. Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; DNA-dependent protein kinase complex; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; enzyme binding; DNA binding; DNA-dependent protein kinase activity; transcription factor binding; ATP binding; protein kinase activity. Biological Process: positive regulation of apoptosis; heart development; germ cell programmed cell death; rhythmic process; T cell differentiation in the thymus; double-strand break repair via nonhomologous end joining; negative regulation of protein amino acid phosphorylation; double-strand break repair; positive regulation of interferon type I production; response to gamma radiation; telomere maintenance; pro-B cell differentiation; somitogenesis; immunoglobulin V(D)J recombination; protein destabilization; B cell lineage commitment; protein modification process; regulation of circadian rhythm; DNA repair; double-strand break repair via homologous recombination; T cell lineage commitment; peptidyl-serine phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; cellular response to insulin stimulus; innate immune response; T cell receptor V(D)J recombination; positive regulation of transcription from RNA polymerase II promoter; brain development. Disease: Immunodeficiency 26 With Or Without Neurologic Abnormalities

0.1 mg

215 USD

0.05 mg

225