Chk1 (Phospho-Ser345) Antibody | MyBioSource MBS857293 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Chk1 (Phospho-Ser345) Antibody

catalog :

MBS857293

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS857293

products type :

Antibody

products full name :

Chk1 (Phospho-Ser345) Antibody

products short name :

Chk1

products name syn :

CHEK1; Chk1; kinase Chk1

other names :

serine/threonine-protein kinase Chk1 isoform 1; Serine/threonine-protein kinase Chk1; serine/threonine-protein kinase Chk1; Chk1-S; checkpoint kinase-1; CHK1 checkpoint homolog; cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; checkpoint kinase 1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1

products gene name :

Chk1

other gene names :

CHEK1; CHEK1; CHK1; CHK1

uniprot entry name :

CHK1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

476

specificity :

Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

50ug/50ul

storage stability :

Store at -20 degree C for 1 year

tested application :

Immunohistochemistry (IHC)

app notes :

IHC: 1:50~1:100

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-SP-Q-P). All Sites: Human: Ser345; Mouse: Ser345; Rat: Ser345. Pathway: CellCycle, Kinase/Phosphatases

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Shiromizu T, et al. (2006) Genes CellsMay: 11(5): 477-85. Hang YW, et al. (2005) Mol Cell: 19(5): 607-18. Lu X, et al. (2005) Genes Dev: 19(10): 1162-74. Falck J, et al. (2005) Nature: 434(7033): 605-11.

ncbi gi num :

166295194

ncbi acc num :

NP_001107593.1

ncbi gb acc num :

NM_001114121.2

uniprot acc num :

O14757

ncbi mol weight :

50,415 Da

ncbi pathways :

Activation Of ATR In Response To Replication Stress Pathway (105754); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway (105752); Circadian Rhythm Pathway (137978); DNA Damage Response Pathway (198788); G1/S DNA Damage Checkpoints Pathway (105740)

ncbi summary :

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

uniprot summary :

Chk1: a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01. Protein type: EC 2.7.11.1; Tumor suppressor; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; CAMKL family; CHK1 subfamily. Chromosomal Location of Human Ortholog: 11q24.2. Cellular Component: nucleoplasm; extracellular space; centrosome; chromosome, telomeric region; condensed nuclear chromosome; intracellular membrane-bound organelle; replication fork; chromatin; cytosol; nucleus. Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding. Biological Process: DNA damage induced protein phosphorylation; chromatin-mediated maintenance of transcription; regulation of mitotic centrosome separation; negative regulation of mitosis; DNA damage checkpoint; peptidyl-threonine phosphorylation; DNA repair; G2/M transition of mitotic cell cycle; G2/M transition DNA damage checkpoint; DNA replication; response to DNA damage stimulus; regulation of cell proliferation

size1 :

0.1 mg

price1 :

215 USD

size2 :

0.05 mg

price2 :

225

more info or order :

MyBioSource product webpage