antibody

CD8

MBS857197

0.1 mL

280 USD

polyclonal

rabbit

nonconjugated

western blot, immunocytochemistry

Antibody

CD8

[CD8]

[T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a]

[T-cell surface glycoprotein CD8 alpha chain isoform 1; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; CD8 antigen, alpha chain; T-cell surface glycoprotein Lyt-2; CD_antigen: CD8a]

[CD8]

[CD8A]

[Cd8a; Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; Lyt-2; Lyt2]

Polyclonal

IgG

Rabbit

Mouse

247

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to near N-terminal residues of Cd8a protein.

Immunogen affinity purified

Liquid; 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol.

100ul, 0.45mg/ml

Store at +4 degree C after thawing. Aliquot store at -20 degree C or -80 degree C. Avoid repeated freeze / thaw cycles.

Western Blot (WB), Immunocytochemistry (ICC)

WB: 1:500

Western Blot

Immunogen: Peptide. Storage Buffer: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Positive control: Mouse Thymus. Cellular Location: Cell membrane. Preservative: 0.05% Sodium Azide.

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated a and ~ subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 is thought to playa role in the process ofT-ceil mediated killing.

124487437

NP_001074579.1

NM_001081110.2

P01731

32-36kDa

Adaptive Immune System Pathway (1110669); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Cell Adhesion Molecules (CAMs) Pathway (83266); Cell Adhesion Molecules (CAMs) Pathway (480); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (1110668); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1110695); Primary Immunodeficiency Pathway (83318)

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

0.1 mL

280 USD