eIF4E (Phospho-Ser209) Antibody | MyBioSource MBS857179 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

eIF4E (Phospho-Ser209) Antibody

catalog :

MBS857179

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS857179

products type :

Antibody

products full name :

eIF4E (Phospho-Ser209) Antibody

products short name :

eIF4E

other names :

eukaryotic translation initiation factor 4E isoform 3; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; eukaryotic translation initiation factor 4E-like 1; eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

products gene name :

eIF4E

other gene names :

EIF4E; EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1; EIF4EL1; EIF4F; eIF-4E; eIF4E

uniprot entry name :

IF4E_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

237

specificity :

elF4E (phospho-Ser209) antibody detects endogenous levels of elF4E only when phosphorylated at serine 209.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Liquid

concentration :

100ul, 0.45mg/ml

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1:500~1:1000, IHC: 1:50~1:100

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T). Pathway: Akt, MAPK, Translation, Insulin/Glucose

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Li BD, et al. (1998) Ann Surg; 227(5): 756-763. Altmann M, et al. (1989) Nucleic Acids Res; 17(18): 7520. De Gregorio E, et al. (2001) RNA; 7(1): 106-113. Gu W, et al. (2004) Nucleic Acids Res; 32(15): 4448-4461

ncbi gi num :

194578907

ncbi acc num :

NP_001124150.1

ncbi gb acc num :

NM_001130678.1

uniprot acc num :

P06730

ncbi mol weight :

32-36kDa

ncbi pathways :

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); Alpha6-Beta4 Integrin Signaling Pathway (198807); Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); BDNF Signaling Pathway (712093); Cap-dependent Translation Initiation Pathway (105967); Cytokine Signaling In Immune System Pathway (366171); Deadenylation Of MRNA Pathway (160994); Deadenylation-dependent MRNA Decay Pathway (160993); Eukaryotic Translation Initiation Pathway (105966); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (105973)

ncbi summary :

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex. Protein type: Translation initiation; Translation. Chromosomal Location of Human Ortholog: 4q23. Cellular Component: mRNA cap complex; stress granule; eukaryotic translation initiation factor 4F complex; cytoplasm; cytosol. Molecular Function: protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding. Biological Process: viral reproduction; translation; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; insulin receptor signaling pathway; translational initiation; gene expression; G1/S transition of mitotic cell cycle; lung development; mRNA catabolic process, deadenylation-dependent decay. Disease: Autism, Susceptibility To, 19

size1 :

0.1 mg

price1 :

215 USD

size2 :

0.05 mg

price2 :

225

more info or order :

MyBioSource product webpage