antibody

IKKa/b (Phospho-Ser180/181) Antibody

MBS857049

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry

Antibody

IKKa/b (Phospho-Ser180/181) Antibody

IKKa/b

inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit alpha; inhibitor of nuclear factor kappa-B kinase subunit alpha; TCF-16; IKK-a kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; transcription factor 16; IkB kinase alpha subunit; Nuclear factor NFkappaB inhibitor kinase alpha; conserved helix-loop-helix ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16

IKKa/b

CHUK; CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha; IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16

IKKA_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

745

IKKa/b antibody detects endogenous levels of IKKa/b only when phosphorylated at serine 180/181.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

50ug/50ul

Store at -20 degree C for 1 year

Western Blot (WB), Immunohistochemistry (IHC)

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:4000

Swiss-Prot No.: O15111/O14920

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human IKKa/b around the phosphorylation site of serine 180/181 (C-T-SP-F-V). Pathway: NF-kappa B, Akt, Cancer/Apoptosis, Kinase/Phosphatases

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Mercurio F., et al. (1997) Science 278:860-866. Ling L., Cao Z., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:3792-3797. Lubin FD, Sweatt JD (2007) Neuron 55, 942-57. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.

62241001

NP_001269.3

NM_001278.3

O15111

AGE/RAGE Pathway (698754); AKT Phosphorylates Targets In The Cytosol Pathway (106475); Activated TLR4 Signalling Pathway (106400); Activation Of NF-kappaB In B Cells Pathway (576251); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Alternative NF-kappaB Pathway (138004)

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541. Protein type: Protein kinase, Other; Kinase, protein; EC 2.7.11.10; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family. Chromosomal Location of Human Ortholog: 10q24-q25. Cellular Component: nucleoplasm; internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; IkappaB kinase complex; cytosol. Molecular Function: protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; ATP binding; protein kinase activity. Biological Process: skeletal muscle contraction; lactation; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; response to toxin; response to lipopolysaccharide; osteoclast differentiation; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; T cell receptor signaling pathway; Rho protein signal transduction; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; morphogenesis of an epithelial sheet; inflammatory response; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; response to drug; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; response to virus; mammary gland epithelial cell proliferation; response to amino acid stimulus; toll-like receptor 2 signaling pathway; odontogenesis of dentine-containing teeth; MyD88-dependent toll-like receptor signaling pathway; response to hydroperoxide; toll-like receptor signaling pathway; striated muscle cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immune response; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation. Disease: Cocoon Syndrome

0.1 mg

215 USD