JAK2 (Phospho-Tyr221) Antibody | MyBioSource MBS857022 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

JAK2 (Phospho-Tyr221) Antibody

catalog :

MBS857022

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS857022

products type :

Antibody

products full name :

JAK2 (Phospho-Tyr221) Antibody

products short name :

JAK2

other names :

tyrosine-protein kinase JAK2; Tyrosine-protein kinase JAK2; tyrosine-protein kinase JAK2; JAK-2; Janus kinase 2 (a protein tyrosine kinase); Janus kinase 2; Janus kinase 2; JAK-2

products gene name :

JAK2

other gene names :

JAK2; JAK2; JTK10; THCYT3; JAK-2

uniprot entry name :

JAK2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

1132

specificity :

JAK2 (phospho-Tyr221) antibody detects endogenous levels of JAK2 only when phosphorylated at tyrosine 221.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

50ug/50ul

storage stability :

Store at -20 degree C for 1 year

tested application :

Immunohistochemistry (IHC)

app notes :

IHC: 1:50~1:100

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 221 (Q-D-YP-H-I). Pathway: Jak/Stat, Cancer/Apoptosis, Akt, Kinase/Phosphatases

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Feener EP, et al.(2004) Mol Cell Biol. 24(11): 4968-4978. Argetsinger LS, et al.(2004) Mol Cell Biol. 24(11): 4955-4967.

ncbi gi num :

4826776

ncbi acc num :

NP_004963.1

ncbi gb acc num :

NM_004972.3

uniprot acc num :

O60674

ncbi mol weight :

130,674 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); BDNF Signaling Pathway (712093); CXCR4-mediated Signaling Events Pathway (137910); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Cholinergic Synapse Pathway (217716); Chromatin Modifying Enzymes Pathway (941403); Chromatin Organization Pathway (941402)

ncbi summary :

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]

uniprot summary :

JAK2: a non-receptor tyrosine-kinase involved in a specific subset of cytokine receptor signaling pathways, including IL-3, -5 and GM-CSF. Interacts with IL23R, SKB1 and STAM2. It has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. Fusion of Jak2 to TEL1 (ETV6) by t(9;12)(p24;p13) causes myeloproliferative disease in humans and mouse models. The Jak inhibitor AG490 inhibits constitutive Jak2 phosphorylation and causes apoptosis in cells from breast cancer and relapsing acute lymphoblastic leukemia. A single activating mutation is associated with several hematological malignancies. Inhibitor: AG490. Protein type: Oncoprotein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Kinase, protein; Protein kinase, TK; TK group; JakA family. Chromosomal Location of Human Ortholog: 9p24. Cellular Component: nucleoplasm; extrinsic to internal side of plasma membrane; cytoskeleton; nuclear matrix; cytoplasm; caveola; cytosol; nucleus; lipid raft. Molecular Function: protein C-terminus binding; histone binding; non-membrane spanning protein tyrosine kinase activity; acetylcholine receptor binding; protein kinase binding; interleukin-12 receptor binding; protein kinase activity; protein binding; growth hormone receptor binding; peptide hormone receptor binding; insulin receptor substrate binding; protein-tyrosine kinase activity; heme binding; phosphoinositide 3-kinase binding; type 1 angiotensin receptor binding; SH2 domain binding; ATP binding; receptor binding. Biological Process: establishment and/or maintenance of chromatin architecture; positive regulation of nitric oxide biosynthetic process; activation of MAPKK activity; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; tyrosine phosphorylation of JAK2 protein; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of apoptosis; response to antibiotic; elevation of cytosolic calcium ion concentration; tumor necrosis factor-mediated signaling pathway; erythrocyte differentiation; mesoderm development; negative regulation of neuron apoptosis; positive regulation of DNA binding; positive regulation of cell activation; positive regulation of protein import into nucleus, translocation; axon regeneration; positive regulation of insulin secretion; JAK-STAT cascade; positive regulation of tumor necrosis factor production; tyrosine phosphorylation of Stat5 protein; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; positive regulation of peptidyl-tyrosine phosphorylation; mineralocorticoid receptor signaling pathway; response to hydroperoxide; tyrosine phosphorylation of Stat1 protein; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of cell differentiation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; hormone-mediated signaling; negative regulation of heart contraction; apoptosis; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; signal transduction; host programmed cell death induced by symbiont; negative regulation of cell proliferation; actin filament polymerization; positive regulation of cell proliferation; cell differentiation; STAT protein nuclear translocation; caspase activation; cell migration; cytokine and chemokine mediated signaling pathway; negative regulation of DNA binding; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; positive regulation of interleukin-1 beta production; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of inflammatory response; innate immune response; negative regulation of cell-cell adhesion; cell motility; blood coagulation; induction of apoptosis by oxidative stress; positive regulation of inflammatory response; positive regulation of cell migration. Disease: Polycythemia Vera; Myelofibrosis; Budd-chiari Syndrome; Erythrocytosis, Familial, 1; Thrombocythemia 3

size1 :

0.1 mg

price1 :

215 USD

size2 :

0.05 mg

price2 :

225

more info or order :

MyBioSource product webpage