antibody

S6 Ribosomal Protein (Phospho-Ser235) Antibody

MBS857008

0.05 mg

225 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunocytochemistry

Antibody

S6 Ribosomal Protein (Phospho-Ser235) Antibody

S6 Ribosomal Protein

40S ribosomal protein S6; Phosphoprotein NP33; RPS6; RS6; ribosomal protein S6

40S ribosomal protein S6; 40S ribosomal protein S6; 40S ribosomal protein S6; phosphoprotein NP33; ribosomal protein S6; Phosphoprotein NP33

RPS6; RPS6; S6

RS6_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

249

S6 Ribosomal protein (phospho-Ser235) antibody detects endogenous levels of S6 Ribosomal protein only when phosphorylated at serine 235.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

50ug/50ul

Store at -20 degree C for 1 year

Western Blot (WB), Immunofluorescence (IF)

WB: 1:500~1:1000

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 235 (R-L-SP-S-L). All Sites: Human: Ser235; Mouse: Ser235; Rat: Ser235. Pathway: Cancer/Apoptosis, Akt, Chromatin/Transcription, Insulin/Glucose

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

McBride K, et al. (1998) Mol Cell Biol 18(9): 5073-5081. Williams AJ, et al. (2003) Plant Physiol 132(4): 2086-2097. Wilson MA, et al. (1997) Biochem J 325(Pt 1): 217-222. Arnesen T, et al. (2005) Biochem J 386(Pt 3): 433-443.

17158044

NP_001001.2

NM_001010.2

P62753

28,681 Da

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway 105970!!B Cell Receptor Signaling Pathway 198909!!BDNF Signaling Pathway 712093!!Cap-dependent Translation Initiation Pathway 105967!!Cytoplasmic Ribosomal Proteins Pathway 198853!!Disease Pathway 530764!!ErbB1 Downstream Signaling Pathway 138057!!Eukaryotic Translation Elongation Pathway 105976!!Eukaryotic Translation Initiation Pathway 105966!!Eukaryotic Translation Termination Pathway 105978

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

0.05 mg

225 USD