CDK2 (Phospho-Thr160) Antibody | MyBioSource MBS856878 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CDK2 (Phospho-Thr160) Antibody

catalog :

MBS856878

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS856878

products type :

Antibody

products full name :

CDK2 (Phospho-Thr160) Antibody

products short name :

CDK2

other names :

cyclin-dependent kinase 2 isoform 3; Cyclin-dependent kinase 2; cyclin-dependent kinase 2; p33 protein kinase; cdc2-related protein kinase; cell division protein kinase 2; cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase

products gene name :

CDK2

other gene names :

CDK2; CDK2; CDKN2; p33(CDK2); CDKN2

uniprot entry name :

CDK2_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

238

specificity :

CDK2 (phospho-Thr160) antibody detects endogenous levels of CDK2 only when phosphorylated at threonine 160.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

50ug/50ul

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:4000

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human CDK2 around the phosphorylation site of threonine 160 (T-Y-TP-H-E). Pathway: Cancer/Apoptosis, CellCycle, Cytoskeletal/Adhesion, Kinase/Phosphatases

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6. Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53. Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56. Liu Y, et al.(2004) J Biol Chem; 279(6): 4507-14.

ncbi gi num :

589811557

ncbi acc num :

NP_001277159.1

ncbi gb acc num :

NM_001290230.1

uniprot acc num :

P24941

ncbi mol weight :

30,035 Da

ncbi pathways :

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); Activation Of ATR In Response To Replication Stress Pathway (105754); Activation Of The Pre-replicative Complex Pathway (105777); B Cell Receptor Signaling Pathway (198909); BARD1 Signaling Events Pathway (137959); CDK-mediated Phosphorylation And Removal Of Cdc6 Pathway (105788); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811)

ncbi summary :

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

uniprot summary :

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276. Protein type: Protein kinase, CMGC; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Cell cycle regulation; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: Cajal body; centrosome; chromosome, telomeric region; X chromosome; condensed chromosome; cytosol; nucleoplasm; Y chromosome; transcription factor complex; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; endosome. Molecular Function: protein binding; cyclin binding; cyclin-dependent protein kinase activity; metal ion binding; histone kinase activity; ATP binding. Biological Process: G1 DNA damage checkpoint; meiosis; mitosis; histone phosphorylation; positive regulation of transcription, DNA-dependent; DNA repair; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; Ras protein signal transduction; positive regulation of cell proliferation; mitotic cell cycle; blood coagulation; DNA replication; G2/M transition of mitotic cell cycle; centrosome duplication; potassium ion transport; positive regulation of DNA replication initiation; G1/S transition of mitotic cell cycle

size1 :

0.1 mg

price1 :

215 USD

size2 :

0.05 mg

price2 :

225

more info or order :

MyBioSource product webpage