eIF2 alpha (Ab-51) Antibody | MyBioSource MBS856716 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

eIF2 alpha (Ab-51) Antibody

catalog :

MBS856716

quantity :

0.05 mg

price :

180 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS856716

products type :

Antibody

products full name :

eIF2 alpha (Ab-51) Antibody

products short name :

eIF2 alpha

products name syn :

eIF2a (Ab-51) Antibody

other names :

eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha

products gene name :

eIF2

other gene names :

EIF2S1; EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha

uniprot entry name :

IF2A_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

315

specificity :

eIF2alpha (Ab-51) antibody detects endogenous levels of total eIF2alpha protein.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4,150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

50ug/50ul

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1:500~1:1000, IHC: 1:50~1:100

other info2 :

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human eIF2alpha around the phosphorylation site of serine 51 (E-L-S^P-R-R). Pathway: NF-kappa B, Translation, Insulin/Glucose

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Xavier Saelens, et,al. (2001) J. Biol. Chem; 276: 41620 - 41628. Hiroyuki Kubota,et,al.(2003) J. Biol. Chem ; 278: 20457 - 20460. Shijian Chu, et,al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992. Eileen Connolly, et,al.(2006) Mol. Cell. Biol ; 26: 3955 - 3965.

ncbi gi num :

4758256

ncbi acc num :

NP_004085.1

ncbi gb acc num :

NM_004094.4

uniprot acc num :

P05198

ncbi mol weight :

36,112 Da

ncbi pathways :

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); BDNF Signaling Pathway (712093); Cap-dependent Translation Initiation Pathway (105967); Eukaryotic Translation Initiation Pathway (105966); Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway (105969); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (105973); Gene Expression Pathway (105937); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873)

ncbi summary :

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

uniprot summary :

eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections. Protein type: Translation initiation; RNA-binding; Translation. Chromosomal Location of Human Ortholog: 14q23.3. Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex. Molecular Function: protein binding; translation initiation factor activity; ribosome binding. Biological Process: regulation of translation initiation in response to stress; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression

size1 :

0.05 mg

price1 :

180 USD

size2 :

0.1 mg

price2 :

215

more info or order :

MyBioSource product webpage