antibody

PKC delta (Ab-645) Antibody

MBS856672

0.05 mg

180 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

Antibody

PKC delta (Ab-645) Antibody

PKC delta

PKCd (Ab-645) Antibody

protein kinase C delta type; Protein kinase C delta type; protein kinase C delta type; protein kinase C delta VIII; tyrosine-protein kinase PRKCD; protein kinase C, delta; Tyrosine-protein kinase PRKCD (EC:2.7.10.2); nPKC-deltaCleaved into the following 2 chains:Protein kinase C delta type regulatory subunit; Protein kinase C delta type catalytic subunitAlternative name(s):Sphingosine-dependent protein kinase-1; SDK1

PKC

PRKCD; PRKCD; MAY1; PKCD; CVID9; nPKC-delta; SDK1

KPCD_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

676

PKCdelta (Ab-645) antibody detects endogenous levels of total PKCdelta protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

50ug/50ul

Store at -20 degree C for 1 year

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

WB: 1:500~1:1000, IHC: 1:50~1:100

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human PKCdelta around the phosphorylation site of serine 645 (R-L-SP-Y-S). Pathway: MAPK, Cancer/Apoptosis, Cytoskeletal/Adhesion, Kinase/Phosphatases

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Kei Sakamoto, et,al. (2003) Am J Physiol Endocrinol Metab, ; 285: E1081 - E1088. Ling Zhang, et,al. (2004) J. Biol. Chem ; 279: 28315 - 28319. Kristof Van Kolen et,al. (2006) FEBS J ; 273: 1843 - 1854. Martin Villalba, et,al. (2002) J. Cell Biol ; 157: 253.

31377782

NP_006245.2

NM_006254.3

Q05655

80,953 Da

AGE/RAGE Pathway (698754); Alpha-synuclein Signaling Pathway (137913); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); B Cell Receptor Signaling Pathway (198909); BDNF Signaling Pathway (712093); Ca-dependent Events Pathway (106497); CaM Pathway (106468)

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

PKCD: an AGC kinase of the PKC family. A novel PKC: Ca2 independent but still regulated by PS, DAG and phorbol esters. Caspase-3 appears to liberate a sphingosine-activated catalytic fragment that has altered regulatory mechanisms and substrate specificities. Protein type: Protein kinase, AGC; EC 2.7.11.13; Tumor suppressor; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Nuclear receptor co-regulator; AGC group; PKC family; Delta subfamily. Chromosomal Location of Human Ortholog: 3p21.31. Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; plasma membrane; intercellular junction; cytosol; nucleus. Molecular Function: protein serine/threonine kinase activity; calcium-independent protein kinase C activity; protein binding; protein kinase C activity; enzyme binding; insulin receptor substrate binding; metal ion binding; enzyme activator activity; non-membrane spanning protein tyrosine kinase activity; ATP binding. Biological Process: B cell proliferation; negative regulation of MAP kinase activity; peptidyl-tyrosine phosphorylation; interleukin-12 production; nerve growth factor receptor signaling pathway; apoptosis; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of protein amino acid dephosphorylation; negative regulation of filopodium formation; positive regulation of superoxide release; regulation of receptor activity; negative regulation of protein binding; cell structure disassembly during apoptosis; epidermal growth factor receptor signaling pathway; negative regulation of peptidyl-tyrosine phosphorylation; platelet activation; fibroblast growth factor receptor signaling pathway; neutrophil activation; protein stabilization; negative regulation of actin filament polymerization; cytokine and chemokine mediated signaling pathway; peptidyl-threonine phosphorylation; cell cycle; phospholipase C activation; negative regulation of inflammatory response; defense response to bacterium; interleukin-10 production; innate immune response; gene expression; immunoglobulin mediated immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway; induction of apoptosis by oxidative stress. Disease: Autoimmune Lymphoproliferative Syndrome, Type Iii

0.05 mg

180 USD

0.1 mg

215