Pim-1 (Phospho-Tyr309) Antibody | MyBioSource MBS856273 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Pim-1 (Phospho-Tyr309) Antibody

catalog :

MBS856273

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS856273

products type :

Antibody

products full name :

Pim-1 (Phospho-Tyr309) Antibody

products short name :

Pim-1

products name syn :

EC 2.7.11.1; PIM-1; Proto-oncogene serine/threonine-protein kinase pim-1

other names :

serine/threonine-protein kinase pim-1 isoform 1; Serine/threonine-protein kinase pim-1; serine/threonine-protein kinase pim-1; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1; Pim-1 proto-oncogene, serine/threonine kinase

products gene name :

Pim-1

other gene names :

PIM1; PIM1; PIM

uniprot entry name :

PIM1_HUMAN

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

404

specificity :

Pim-1 (Phospho-Tyr309) antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine 309.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB: 1:500~1:1000, ELISA: 1:40000

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Pim-1 around the phosphorylation site of tyrosine 309 (H-R-YP-H-G). All Sites: Human: Tyr309; Mouse: Tyr302; Rat: Tyr218. Pathway: Phospho-specific

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Selten G., Cell 46:603-611(1986). Saris C.J., EMBO J. 10:655-664(1991). Maita H., Eur. J. Biochem. 267:5168-5178(2000).

ncbi gi num :

342307046

ncbi acc num :

NP_001230115.1

ncbi gb acc num :

NM_001243186.1

uniprot acc num :

P11309

ncbi mol weight :

35,686 Da

ncbi pathways :

Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); C-MYB Transcription Factor Network Pathway (138073); IL-5 Signaling Pathway (198770); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Role Of Calcineurin-dependent NFAT Signaling In Lymphocytes Pathway (137953); Validated Targets Of C-MYC Transcriptional Activation Pathway (169351)

ncbi summary :

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]

uniprot summary :

Pim1: a proto-oncogene serine/threonine kinase involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerced by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl- X(L)/BCL2L1. Phosphorylation of ASK1 an other proapoptotic protein, by PIM1, significantly decreases ASK1 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of p21Cip1, a regulator of cell cycle progression at G1, results in the relocation of p21Cip1 to the cytoplasm and enhanced p21Cip1 protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, p27Kip1, at both transcriptional and post- translational levels. Phosphorylation of p27Kip1,induces 14-3-3- proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Binds to RP9. Isoform 1, but not isoform 2, binds BMX. Isoform 2 interacts with p27Kip1 and FOXO3. Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM1, ubiquitination and proteasomal degradation. Interacts with HSP90, this interaction stabilizes PIM1 protein levels. Ubiquitinated form interacts with HSP70 and promotes its proteosomal degradation. Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines. Induced by different cellular stresses, heat shock and cytotoxic agents. Expressed primarily in cells of the hematopoietic and germline lineages. 2 isoforms of the human protein are produced by alternative initiation. Both isoforms are expressed in prostate cancer cell lines. Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); CAMK group; PIM family. Chromosomal Location of Human Ortholog: 6p21.2. Cellular Component: cytoplasm; plasma membrane; nucleus. Molecular Function: protein serine/threonine kinase activity; protein binding; manganese ion binding; transcription factor binding; ATP binding; ribosomal small subunit binding. Biological Process: cell proliferation; apoptosis; protein amino acid autophosphorylation; multicellular organismal development; negative regulation of transcription factor activity; regulation of mitotic cell cycle; cell cycle; hyaluronan metabolic process; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; negative regulation of apoptosis

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage