IRF-3 (Phospho-Ser385) Antibody | MyBioSource MBS856015 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

IRF-3 (Phospho-Ser385) Antibody

catalog :

MBS856015

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS856015

products type :

Antibody

products full name :

IRF-3 (Phospho-Ser385) Antibody

products short name :

IRF-3

products name syn :

Interferon regulatory factor 3; IRF3

other names :

interferon regulatory factor 3 isoform 2; Interferon regulatory factor 3; interferon regulatory factor 3; interferon regulatory factor 3

products gene name :

IRF-3

other gene names :

IRF3; IRF3; IRF-3

uniprot entry name :

IRF3_HUMAN

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

452

specificity :

IRF-3 (Phospho-Ser385) antibody detects endogenous levels of IRF-3 only when phosphorylated at serine 385.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

app notes :

WB: 1:500~1:1000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:10000

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human IRF-3 around the phosphorylation site of serine 385 (G-A-SP-S-L). All Sites: Human: Ser385; Mouse: Ser387; Rat: Ser380. Pathway: Phospho-specific

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Au W.W.-C., Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995). The MGC Project Team; Genome Res. 14:2121-2127(2004). Bellingham J., Ann. Hum. Genet. 62:231-234(1998).

ncbi gi num :

308199446

ncbi acc num :

NP_001184051.1

ncbi gb acc num :

NM_001197122.1

uniprot acc num :

Q14653

ncbi mol weight :

12,330 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (106400); Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway (187088); Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Apoptosis Pathway (198797); Cytokine Signaling In Immune System Pathway (366171); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115)

ncbi summary :

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

uniprot summary :

IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; negative regulation of interferon-beta biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of defense response to virus by host; toll-like receptor 3 signaling pathway; response to exogenous dsRNA; toll-like receptor signaling pathway; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon-beta production; innate immune response; interferon type I biosynthetic process; toll-like receptor 4 signaling pathway; response to DNA damage stimulus; defense response to virus; negative regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of cytokine secretion. Disease: Herpes Simplex Encephalitis, Susceptibility To, 7

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage