antibody

GAD65 Antibody

MBS855593

0.1 mg

290 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry

Antibody

GAD65 Antibody

GAD65

Homo sapiens glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) (GAD2), mRNA; Glutamate decarboxylase 2; glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

GAD65

GAD2; GAD2; GAD65; GAD65; GAD-65

DCE2_HUMAN

Polyclonal

Rabbit

Human

2457

Lyophilized anti-GAD65 antibody purified by peptide affinity column containing 1% BSA and 0.02% NaN3

100ug/200ul

Already reconstituted in 200ul sterile H2O. The antibody is stable for at least one year when stored at -20 degree C after reconstitution. Avoid repeated freeze/defrost cycles.

Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA titer: 1:40,000-1:80,000. Western blot analysis: 1:200-500. Immunohistochemistry: 1:50-200. Optimal dilutions/concentrations should be determined by the end user.

Source Note: Rabbit polyclonal GAD65 (1) antibody was raised against a 15 amino acid peptide from near the N terminal residues of human GAD65 protein. Reactivity Note: Not yet tested in other species.

Pathway: glutamate decarboxylase 2

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

4503874

NP_000809.1

NM_000818.1

65,411 Da

Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Biogenic Amine Synthesis Pathway (198793); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); GABA (gamma-Aminobutyrate) Shunt Pathway (413433); GABA (gamma-Aminobutyrate) Shunt Pathway (468220); GABA Synthesis Pathway (187175); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174)

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family. Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Lyase; EC 4.1.1.15. Chromosomal Location of Human Ortholog: 10p11.23. Cellular Component: presynaptic membrane; Golgi membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; plasma membrane; cell junction; cytosol. Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding. Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process

0.1 mg

290 USD