MAP2K7 (Phospho-Thr275) Antibody | MyBioSource MBS854995 product information

product summary

company name :

MyBioSource

product type :

other

product name :

MAP2K7 (Phospho-Thr275) Antibody

catalog :

MBS854995

quantity :

0.1 mg

price :

280 USD

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using MAP2K7 (Phospho-Thr275) antibody.

product information

catalog number :

MBS854995

products type :

Antibody

products full name :

MAP2K7 (Phospho-Thr275) Antibody

products short name :

[MAP2K7]

products name syn :

[c-Jun N-terminal kinase kinase 2; Dual specificity mitogen-activated protein kinase kinase 7; EC 2.7.12.2; JNK activating kinase 2; JNK kinase 2; JNKK 2; JNKK2; kinase MKK7; MAP kinase kinase 7; MAP2K7; MAPK/ERK kinase 7; MAPKK 7; MP2K7; PRKMK7]

other names :

[dual specificity mitogen-activated protein kinase kinase 7 isoform 1; Dual specificity mitogen-activated protein kinase kinase 7; dual specificity mitogen-activated protein kinase kinase 7; mitogen-activated protein kinase kinase 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; Stress-activated protein kinase kinase 4; SAPK kinase 4; SAPKK-4; SAPKK4; c-Jun N-terminal kinase kinase 2; JNK kinase 2; JNKK 2]

products gene name :

[MAP2K7]

other gene names :

[MAP2K7; MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4; JNKK2; MEK7; MKK7; PRKMK7; SKK4; MAP kinase kinase 7; MAPKK 7; MEK 7; SAPK kinase 4; SAPKK-4; SAPKK4; JNK kinase 2; JNKK 2]

reactivity :

Human, Mouse, Rat

sequence length :

435

specificity :

MAP2K7 (Phospho-Thr275) antibody detects endogenous levels of MAP2K7 only when phosphorylated at threonine 275.

purity :

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

form :

Rabbit IgG in phosphate buffered saline (without, Mg 2+ and Ca 2+ ), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at-20 degree C for 1 year.

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB: 1:500~1:1000. ELISA: 1:20000

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MAP2K7 around the phosphorylation site of threonine 275 (A-K-TP-R-S).

other info2 :

All Sites: Human: Thr275. Mouse: Thr291. Rat: Thr275

products categories :

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

products references :

Wu Z., Mol. Cell. Biol. 17:7407-7416(1997). Lu X., J. Biol. Chem. 272:24751-24754(1997). The MGC Project Team; Genome Res. 14:2121-2127(2004).

ncbi gi num :

662033893

ncbi acc num :

NP_001284484.1

ncbi gb acc num :

NM_001297555.1

uniprot acc num :

O14733

ncbi mol weight :

48,182 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (1269236); CDC42 Signaling Events Pathway (137994); Cellular Senescence Pathway (1270426); Cellular Responses To Stress Pathway (1270414); Cellular Roles Of Anthrax Toxin Pathway (138076); Disease Pathway (1268854); EGFR1 Signaling Pathway (198782); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); ErbB Signaling Pathway (198844)

ncbi summary :

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

uniprot summary :

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.

size1 :

0.1 mg

price1 :

280 USD

more info or order :

MyBioSource product webpage