antibody

EPHA2/3 (Phospho-Tyr588/596) Antibody

MBS854845

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunocytochemistry, enzyme immunoassay

Antibody

EPHA2/3 (Phospho-Tyr588/596) Antibody

EPHA2/3

EC 2.7.10.1; ECK; Ephrin type-A receptor 2 precursor; Epithelial cell kinase; MPK-5; SEK-2; SEK2; Tyrosine-protein kinase receptor ECK; kinase EphA2

ephrin type-A receptor 2; Ephrin type-A receptor 2; ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase; EPH receptor A2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK

EPHA2/3

EPHA2; EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6; ECK

EPHA2_HUMAN

Rabbit

Human, Mouse

976

EPHA2/3/4 (Phospho-Tyr588/596) antibody detects endogenous levels of EPHA2/3/4 only when phosphorylated at tyrosine 588/596.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)

WB: 1:500~1:1000, IF: 1:100~1:500, ELISA: 1:20000

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human EPHA2/3/4 around the phosphorylation site of tyrosine 588/596 (T-YP-V-D-P). All Sites: Human: Tyr588/Tyr596/Tyr596; Mouse: Tyr589/Tyr596/Tyr596. Pathway: Phospho-specific

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Lindberg R.A., Mol. Cell. Biol. 10:6316-6324(1990). Zhang Y., Mol. Cell. Proteomics 4:1240-1250(2005). Zhuang G., J. Biol. Chem. 282:2683-2694(2007).

32967311

NP_004422.2

NM_004431.3

P29317

54,305 Da

Arf6 Signaling Events Pathway (138034); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Direct P53 Effectors Pathway (137939); EPH-Ephrin Signaling Pathway (1016174); EPH-ephrin Mediated Repulsion Of Cells Pathway (1016178); EPHA Forward Signaling Pathway (138041); EPHA-mediated Growth Cone Collapse Pathway (1016175)

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]

EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice. Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; Eph family. Chromosomal Location of Human Ortholog: 1p36. Cellular Component: cell surface; focal adhesion; integral to plasma membrane; plasma membrane. Molecular Function: protein binding; ephrin receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding. Biological Process: neural tube development; axial mesoderm formation; axon guidance; cell migration; peptidyl-tyrosine phosphorylation; negative regulation of protein kinase B signaling cascade; viral reproduction; multicellular organismal development; notochord formation; mammary gland epithelial cell proliferation; osteoclast differentiation; regulation of blood vessel endothelial cell migration; bone remodeling; regulation of angiogenesis; osteoblast differentiation; keratinocyte differentiation; regulation of cell adhesion mediated by integrin; DNA damage response, signal transduction resulting in induction of apoptosis; notochord cell development; protein kinase B signaling cascade; ephrin receptor signaling pathway; angiogenesis; cell adhesion; vasculogenesis; skeletal development. Disease: Cataract 6, Multiple Types

0.1 mg

215 USD