GRP78 Antibody | MyBioSource MBS854638 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GRP78 Antibody

catalog :

MBS854638

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS854638

products type :

Antibody

products full name :

GRP78 Antibody

products short name :

GRP78

products name syn :

BIP; GLUCOSE-REGULATED PROTEIN 78-KD; HEAT-SHOCK 70-KD PROTEIN 5; IMMUNOGLOBULIN HEAVY CHAIN-BINDING PROTEIN; heat shock 70kDa protein 5 (glucose-regulated protein; 78kDa)

other names :

78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; epididymis secretory sperm binding protein Li 89n; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

products gene name :

GRP78

other gene names :

HSPA5; HSPA5; BIP; MIF2; GRP78; HEL-S-89n; GRP78; GRP-78; BiP

uniprot entry name :

GRP78_HUMAN

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

654

specificity :

GRP78 antibody detects endogenous levels of total GRP78 protein.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

100ug/100ul

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

app notes :

WB: 1:500~1:1000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:5000

other info2 :

Immunogen: The antiserum was produced against synthesized peptide derived from human GRP78. Pathway: Intermediates

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Uma K. Misra, J. Biol. Chem., Oct 2002; 277: 42082 - 42087. CA Clairmont, J. Biol. Chem., Feb 1992; 267: 3983 - 3990. J Tooze, J. Cell Biol., Jul 1989; 109: 35. Jong K. Yun, PNAS, Dec 1997; 94: 13903.

ncbi gi num :

16507237

ncbi acc num :

NP_005338.1

ncbi gb acc num :

NM_005347.4

uniprot acc num :

P11021

ncbi mol weight :

72,333 Da

ncbi pathways :

ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); Adaptive Immune System Pathway (366160); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Hemostasis Pathway (106028); IRE1alpha Activates Chaperones Pathway (105906); Immune System Pathway (106386)

ncbi summary :

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

uniprot summary :

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Protein type: Heat shock protein; Chaperone. Chromosomal Location of Human Ortholog: 9q33.3. Cellular Component: signalosome; endoplasmic reticulum membrane; smooth endoplasmic reticulum; cell surface; focal adhesion; mitochondrion; endoplasmic reticulum lumen; endoplasmic reticulum; ER-Golgi intermediate compartment; membrane; melanosome; plasma membrane; integral to endoplasmic reticulum membrane; midbody; nucleus. Molecular Function: protein domain specific binding; protein binding; enzyme binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; ATPase activity; ribosome binding; calcium ion binding; misfolded protein binding; glycoprotein binding; ATP binding. Biological Process: platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; substantia nigra development; cellular protein metabolic process; platelet degranulation; positive regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; blood coagulation; ER overload response; negative regulation of apoptosis; positive regulation of cell migration

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage