antibody

Vitamin D3 Receptor (Phospho-Ser51) Antibody

MBS854516

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, enzyme immunoassay

Antibody

Vitamin D3 Receptor (Phospho-Ser51) Antibody

Vitamin D3 Receptor

1; 25-dihydroxyvitamin D3 receptor; NR1I1; vitamin D receptor; vitamin D3 receptor

vitamin D3 receptor isoform VDRA; Vitamin D3 receptor; vitamin D3 receptor; 1,25-dihydroxyvitamin D3 receptor; vitamin D nuclear receptor variant 1; nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163; vitamin D (1,25- dihydroxyvitamin D3) receptor; 1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1

VDR; VDR; NR1I1; PPP1R163; NR1I1; VDR

VDR_HUMAN

Rabbit

Human, Mouse, Rat

427

Vitamin D3 Receptor (Phospho-Ser51) antibody detects endogenous levels of Vitamin D3 Receptor only when phosphorylated at serine 51.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation s

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

Western Blot (WB), ELISA (EIA)

WB: 1:500~1:1000, ELISA: 1:20000

Reactivity Note: Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%)

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Vitamin D3 Receptor around the phosphorylation site of serine 51 (R-R-SP-M-K). All Sites: Human: Ser51; Mouse: Ser51; Rat: Ser51. Pathway: Phospho-specific

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Baker A.R., Proc. Natl. Acad. Sci. U.S.A. 85:3294-3298(1988). Goto H., Biochim. Biophys. Acta 1132:103-108(1992). Miyamoto K., Mol. Endocrinol. 11:1165-1179(1997).

4507883

NP_000367.1

NM_000376.2

P11473

53,883 Da

Direct P53 Effectors Pathway (137939); Drug Induction Of Bile Acid Pathway (698755); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848)

This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]

VDR: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. Defects in VDR are the cause of rickets vitamin D- dependent type 2A (VDDR2A). A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. Belongs to the nuclear hormone receptor family. NR1 subfamily. Protein type: Nuclear receptor; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 12q13.11. Cellular Component: nucleoplasm; nucleus; receptor complex. Molecular Function: retinoid X receptor binding; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; vitamin D3 receptor activity; transcription factor activity. Biological Process: lactation; transcription initiation from RNA polymerase II promoter; positive regulation of apoptosis involved in mammary gland involution; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; decidualization; signal transduction; intestinal absorption; cellular calcium ion homeostasis; negative regulation of cell proliferation; calcium ion transport; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; skeletal development; positive regulation of keratinocyte differentiation. Disease: Vitamin D-dependent Rickets, Type 2a; Osteoporosis

0.1 mg

215 USD