antibody

PIP5K Antibody

MBS854145

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunocytochemistry, enzyme immunoassay

Antibody

PIP5K Antibody

PIP5K

1- phosphatidylinositol-4-phosphate 5-kinase; EC 2.7.1.68; FYV1; FYVE finger-containing phosphoinositide kinase; KIAA0981; PIKfyve; PIP5K3; PtdIns(4)P-5- kinase; p235

1-phosphatidylinositol 3-phosphate 5-kinase isoform 4; 1-phosphatidylinositol 3-phosphate 5-kinase; 1-phosphatidylinositol 3-phosphate 5-kinase; PIPkin-III; type III PIP kinase; epididymis luminal protein 37; zinc finger, FYVE domain containing 29; 1-phosphatidylinositol-3-phosphate 5-kinase; phosphatidylinositol 3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III; phosphoinositide kinase, FYVE finger containing; FYVE finger-containing phosphoinositide kinase; PIKfyve; Phosphatidylinositol 3-phosphate 5-kinase type III; PIPkin-III; Type III PIP kinase

PIP5K

PIKFYVE; PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29; KIAA0981; PIP5K3; Phosphatidylinositol 3-phosphate 5-kinase; PIPkin-III; Type III PIP kinase

FYV1_HUMAN

Rabbit

Human, Mouse

548

PIP5K antibody detects endogenous levels of total PIP5K protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)

WB: 1:500~1:1000, IF: 1:100~1:500, ELISA: 1:5000

Immunogen: The antiserum was produced against synthesized peptide derived from internal of human PIP5K. Pathway: Phosphatases/Kinases

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Ota T., Nat. Genet. 36:40-45(2004). Sbrissa D., J. Biol. Chem. 277:6073-6079(2002). Brill L.M., Anal. Chem. 76:2763-2772(2004). ------------------------------------------------------------------------------------------------------------------------------------------

295789162

NP_001171471.1

NM_001178000.1

Q9Y2I7

61,595 Da

3-phosphoinositide Biosynthesis Pathway (545314); 3-phosphoinositide Biosynthesis Pathway (138600); Inositol Phosphate Metabolism Pathway (82987); Inositol Phosphate Metabolism Pathway (362); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PI Metabolism Pathway (645332); Phagosome Pathway (153910); Phagosome Pathway (153859); Phosphatidylinositol Signaling System Pathway (83052)

Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010]

PIKFYVE: The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes. Defects in PIKFYVE are the cause of corneal fleck dystrophy (CFD). CFD is an autosomal dominant disorder of the cornea characterized by numerous small white flecks scattered in all levels of the stroma. Although CFD may occasionally cause mild photophobia, patients are typically asymptomatic and have normal vision. 4 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.7.1.150; Kinase, lipid; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q34. Cellular Component: Golgi membrane; late endosome membrane; perinuclear region of cytoplasm; early endosome membrane; endosome membrane; cytoplasmic vesicle; intercellular junction; cytosol; lipid raft. Molecular Function: protein binding; 1-phosphatidylinositol-3-phosphate 5-kinase activity; 1-phosphatidylinositol-4-phosphate 5-kinase activity; phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity; metal ion binding; ATP binding. Biological Process: receptor-mediated endocytosis; myelin formation; cellular protein metabolic process; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde transport, endosome to Golgi. Disease: Corneal Dystrophy, Fleck

0.1 mg

215 USD