antibody

SirT1 (Ab-47) Antibody

MBS854093

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

SirT1 (Ab-47) Antibody

SirT1

hSIR2; hSIRT1; NAD-dependent deacetylase sirtuin 1; SIR1; SIR2-like protein 1; SIR2L1; sirtuin 1

NAD-dependent protein deacetylase sirtuin-1 isoform b; NAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1; sirtuin 1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2

SirT1

SIRT1; SIRT1; SIR2L1; SIR2L1; hSIRT1; hSIR2; 75SirT1

SIR1_HUMAN

Rabbit

Human, Mouse

452

SirT1 (Ab-47) antibody detects endogenous levels of total SirT1 protein.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:5000

Reactivity Note: Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human SirT1 around the phosphorylation site of serine 47 (E-R-SP-P-G). Pathway: Cancer/Apoptosis

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Rogers J., Nature 429:375-381(2004). Weinberg R.A., Cell 107:149-159(2001). Sinclair D.A., Nature 425:191-196(2003). ------------------------------------------------------------------------------------------------------------------------------------------

215982798

NP_001135970.1

NM_001142498.1

Q96EB6

61,066 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Androgen Receptor Signaling Pathway (198806); Cellular Response To Heat Stress Pathway (980470); Cellular Responses To Stress Pathway (645258); E2F Transcription Factor Network Pathway (137934); Energy Metabolism Pathway (198907); Epigenetic Regulation Of Gene Expression Pathway (980449)

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

SIRT1: an NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separate cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5 -AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. Elevation of NAD(+)/NADP(+) ratio activates SIRT1. Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG. Involved in liver and muscle metabolism. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Widely expressed. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; Deacetylase; Nuclear receptor co-regulator; EC 3.5.1.-. Chromosomal Location of Human Ortholog: 10q21.3. Cellular Component: nucleoplasm; chromatin silencing complex; PML body; mitochondrion; ESC/E(Z) complex; nuclear chromatin; cytoplasm; nucleolus; nuclear inner membrane; nuclear envelope; nuclear heterochromatin; nucleus. Molecular Function: protein C-terminus binding; deacetylase activity; identical protein binding; histone binding; p53 binding; metal ion binding; protein deacetylase activity; transcription factor binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; NAD-dependent histone deacetylase activity; bHLH transcription factor binding; mitogen-activated protein kinase binding; histone deacetylase activity; HLH domain binding; transcription corepressor activity; NAD+ ADP-ribosyltransferase activity. Biological Process: muscle development; establishment and/or maintenance of chromatin architecture; viral reproduction; positive regulation of apoptosis; regulation of mitotic cell cycle; positive regulation of caspase activity; protein ubiquitination; negative regulation of prostaglandin biosynthetic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of gene expression, epigenetic; behavioral response to starvation; positive regulation of adaptive immune response; positive regulation of histone H3-K9 methylation; positive regulation of DNA repair; transcription, DNA-dependent; negative regulation of transcription factor activity; pyrimidine dimer repair via nucleotide-excision repair; cell aging; negative regulation of I-kappaB kinase/NF-kappaB cascade; ovulation from ovarian follicle; cellular response to starvation; negative regulation of fat cell differentiation; protein amino acid ADP-ribosylation; cholesterol homeostasis; regulation of endodeoxyribonuclease activity; methylation-dependent chromatin silencing; inhibition of NF-kappaB transcription factor; maintenance of chromatin silencing; negative regulation of phosphorylation; regulation of protein import into nucleus, translocation; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; triacylglycerol mobilization; negative regulation of transcription, DNA-dependent; peptidyl-lysine acetylation; rRNA processing; negative regulation of apoptosis; chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; establishment of chromatin silencing; chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; response to insulin stimulus; DNA synthesis during DNA repair; protein amino acid deacetylation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; angiogenesis; circadian regulation of gene expression; DNA replication; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; single strand break repair; positive regulation of chromatin silencing; negative regulation of TOR signaling pathway; negative regulation of protein kinase B signaling cascade; protein destabilization; histone deacetylation; DNA repair; regulation of cell proliferation; negative regulation of helicase activity; response to hydrogen peroxide; fatty acid homeostasis; white fat cell differentiation; cell glucose homeostasis; gene expression; spermatogenesis; positive regulation of protein amino acid phosphorylation; positive regulation of insulin receptor signaling pathway; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of macroautophagy; response to DNA damage stimulus

0.1 mg

215 USD