antibody

NOTCH4 (Cleaved-Val1432) Antibody

MBS853994

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, enzyme immunoassay

Antibody

NOTCH4 (Cleaved-Val1432) Antibody

NOTCH4

Neurogenic locus notch homolog protein 4 [Precursor]; Notch 4; hNotch4; Notch 4 extracellular truncation; Notch 4 intracellular domain; NOTCH4; INT3

neurogenic locus notch homolog protein 4 preproprotein; Neurogenic locus notch homolog protein 4; neurogenic locus notch homolog protein 4; Notch homolog 4; notch 4

NOTCH4

NOTCH4; NOTCH4; INT3; INT3; Notch 4; hNotch4

NOTC4_HUMAN

Rabbit

Human

2003

NOTCH4 (Cleaved-Val1432) antibody detects endogenous levels of fragment of activated NOTCH4 resulting from cleavage adjacent to Val1432.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

Western Blot (WB), ELISA (EIA)

WB: 1:500~1:1000, ELISA: 1:1000

Immunogen: The antiserum was produced against synthesized peptide derived from human NOTCH4. Pathway: Cleaved-specific

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Sugaya K., Gene 189:235-244(1997). Li L., Genomics 51:45-58(1998). Gray G.E., Am. J. Pathol. 154:785-794(1999).

55770876

NP_004548.3

NM_004557.3

Q99466

39,617 Da

Delta-Notch Signaling Pathway (198879); Dorso-ventral Axis Formation Pathway (114227); Dorso-ventral Axis Formation Pathway (472); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Neural Crest Differentiation Pathway (672460); Notch Signaling Pathway (198891); Notch Signaling Pathway (83062)

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. This gene may be associated with susceptibility to schizophrenia in a small portion of cases. An alternative splice variant has been described but its biological nature has not been determined. [provided by RefSeq, Jul 2008]

NOTCH4: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4. Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. Belongs to the NOTCH family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: nucleoplasm; Golgi membrane; endoplasmic reticulum membrane; cell surface; integral to plasma membrane; plasma membrane; extracellular region; cytosol; nucleus. Molecular Function: protein binding; protein heterodimerization activity; calcium ion binding; receptor activity. Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; positive regulation of transcription, DNA-dependent; Notch receptor processing; cell fate determination; morphogenesis of a branching structure; activation of Notch receptor target transcription factor; patterning of blood vessels; embryonic development; negative regulation of cell differentiation; mammary gland development; negative regulation of endothelial cell differentiation; gene expression; hemopoiesis; endothelial cell morphogenesis; cell differentiation

0.1 mg

215 USD