antibody

ATF-4 (Phospho-Ser219) Antibody

MBS853958

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, enzyme immunoassay

Antibody

ATF-4 (Phospho-Ser219) Antibody

ATF-4

Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB

cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-dependent transcription factor ATF-4; cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-responsive element-binding protein 2; tax-responsive enhancer element-binding protein 67; activating transcription factor 4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; Tax-responsive enhancer element-binding protein 67; TaxREB67

ATF-4

ATF4; ATF4; CREB2; TXREB; CREB-2; TAXREB67; CREB2; TXREB; cAMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; TaxREB67

ATF4_HUMAN

Rabbit

Human, Mouse

351

ATF-4 (Phospho-Ser219) Antibody detects endogenous levels of ATF4 only when phosphorylated at serine 219.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

Western Blot (WB), ELISA (EIA)

WB: 1:500~1:1000, ELISA: 1:20000

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human ATF4 around the phosphorylation site of serine 219. All Sites: Human: Ser219; Mouse: Ser218. Pathway: Phospho-specific

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Tsujimoto A., J. Virol. 65:1420-1426(1991). Karpinski B.A., Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824(1992). Karpinski B.A., Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824(1992).

33469976

NP_001666.2

NM_001675.4

P18848

38,590 Da

ATF4 Activates Genes Pathway (530772); ATF6-alpha Activates Chaperone Genes Pathway (530770); ATF6-alpha Activates Chaperones Pathway (105905); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Cholinergic Synapse Pathway (217716)

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]

ATF-4: a transcription factor that is a member of the leucine zipper family. Isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). Involved in cisplatin resistance. Other members of the family include AP-1 transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. Two alternatively spliced isoforms have been described. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: nucleoplasm; neuron projection; cytoplasm; microtubule organizing center; nucleus. Molecular Function: protein C-terminus binding; protein binding; leucine zipper domain binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; amino acid metabolic process; positive regulation of apoptosis; unfolded protein response; negative regulation of potassium ion transport; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase I promoter; gluconeogenesis; cellular response to glucose starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; gamma-aminobutyric acid signaling pathway

0.1 mg

215 USD