antibody

PEK/PERK (Phospho-Thr981) Antibody

MBS853840

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

ELISA, immunohistochemistry, enzyme immunoassay

Antibody

PEK/PERK (Phospho-Thr981) Antibody

PEK/PERK

E2AK3; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2-alpha kinase 3 precursor; HsPEK; kinase PERK; Pancreatic eIF2-alpha kinase; PEK; PERK; PRKR-like endoplasmic reticulum kinase

eukaryotic translation initiation factor 2-alpha kinase 3; Eukaryotic translation initiation factor 2-alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; hsPEK; pancreatic EIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase; eukaryotic translation initiation factor 2 alpha kinase 3; eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK

PEK/PERK

EIF2AK3; EIF2AK3; PEK; WRS; PERK; PEK; PERK; HsPEK

E2AK3_HUMAN

Rabbit

Human, Mouse, Rat

1116

PEK/PERK (Phospho-Thr981) antibody detects endogenous levels of PEK/PERK only when phosphorylated at threonine 981.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

Immunohistochemistry (IHC), ELISA (EIA)

IHC: 1:50~1:100, ELISA: 1:40000

Reactivity Note: Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 92%); Rat (Identities = 92%, Positives = 92%)

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PEK/PERK around the phosphorylation site of threonine 981 (R-H-TP-G-Q). All Sites: Human: Thr981; Mouse: Thr980; Rat: Thr974. Pathway: Phospho-specific

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Shi Y., An J., J. Biol. Chem. 274:5723-5730(1999). Sood R., Biochem. J. 346:281-293(2000). The MGC Project Team; Genome Res. 14:2121-2127(2004).

134304838

NP_004827.4

NM_004836.5

Q9NZJ5

125,216 Da

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Influenza A Pathway (217173)

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010]

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK. Protein type: Translation; Kinase, protein; Membrane protein, integral; Protein kinase, Other; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; PEK family; PEK subfamily. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytoplasm; integral to endoplasmic reticulum membrane. Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; enzyme binding; protein phosphatase binding; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity. Biological Process: lactation; fat cell differentiation; translation; positive regulation of protein binding; protein amino acid autophosphorylation; protein amino acid phosphorylation; bone mineralization; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; regulation of fatty acid metabolic process; angiogenesis; skeletal development; ER overload response; protein homooligomerization; endoplasmic reticulum organization and biogenesis; caspase activation; ossification; negative regulation of myelination; unfolded protein response; calcium-mediated signaling; chondrocyte development; SREBP-mediated signaling pathway; endocrine pancreas development; peptidyl-serine phosphorylation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; insulin-like growth factor receptor signaling pathway; insulin secretion; negative regulation of translation; negative regulation of translation initiation in response to stress; negative regulation of apoptosis. Disease: Epiphyseal Dysplasia, Multiple, With Early-onset Diabetes Mellitus

0.1 mg

215 USD