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AXL (Phospho-Tyr691) Antibody

MBS853818

0.1 mg

280 USD

Antibody

AXL (Phospho-Tyr691) Antibody

[AXL]

[Adhesion-related kinase; ARK; AXL oncogene; EC 2.7.10.1; kinase AXL; Tyrosine-protein kinase receptor UFO precursor; UFO]

[tyrosine-protein kinase receptor UFO isoform 2; Tyrosine-protein kinase receptor UFO; tyrosine-protein kinase receptor UFO; AXL receptor tyrosine kinase; AXL oncogene]

[AXL]

[AXL; AXL; ARK; UFO; JTK11; Tyro7; UFO]

Human, Mouse, Rat

885

AXL (Phospho-Tyr691) antibody detects endogenous levels of AXL only when phosphorylated at tyrosine 691.

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at-20 degree C for 1 year.

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

WB: 1:500~1:1000. IHC: 1:50~1:100. ELISA: 1:20000

Testing Data

Immunohistochemistry (IHC)

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human AXL around the phosphorylation site of tyrosine 691 (K-I-YP-N-G).

All Sites: Human: Tyr691. Mouse: Tyr692. Rat: Tyr692

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

Partanen J., Proc. Natl. Acad. Sci. U.S.A. 87:8913-8917(1990). Janssen J.W.G., Oncogene 6:2113-2120(1991). O'Bryan J.P., Mol. Cell. Biol. 11:5016-5031(1991).

21536468

NP_001690.2

NM_001699.5

P30530

97,378 Da

The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.

0.1 mg

280 USD