4E-BP1 (Phospho-Ser64) Antibody | MyBioSource MBS853497 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

4E-BP1 (Phospho-Ser64) Antibody

catalog :

MBS853497

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS853497

products type :

Antibody

products full name :

4E-BP1 (Phospho-Ser64) Antibody

products short name :

4E-BP1

products name syn :

4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6; PHAS-1; PHAS-I; 4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6; PHAS-2; PHAS-I

other names :

eukaryotic translation initiation factor 4E-binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1; eukaryotic translation initiation factor 4E binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

products gene name :

4E-BP1

other gene names :

EIF4EBP1; EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I; 4E-BP1; eIF4E-binding protein 1; PHAS-I

uniprot entry name :

4EBP1_HUMAN

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

118

specificity :

4E-BP1 (Phospho-Ser64) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at serine 64.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

100ug/100ul

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

app notes :

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:1000

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of serine 64 (R-N-SP-P-V). All Sites: Human: Ser64; Mouse: Ser63; Rat: Ser63. Pathway: Phospho-specific

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Anne-Claude Gingras, Genes & Dev., Jun 1999; 13: 1422. Guangming Liu, J. Biol. Chem., Mar 2002; 277: 8810 - 8816. Ya-Ping Jiang, J. Biol. Chem., Mar 2001; 276: 10943 - 10951. Anne-Claude Gingras, Genes & Dev., Feb 1998; 12: 502.

ncbi gi num :

4758258

ncbi acc num :

NP_004086.1

ncbi gb acc num :

NM_004095.3

uniprot acc num :

Q13541

ncbi mol weight :

12,580 Da

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Alpha6-Beta4 Integrin Signaling Pathway (198807); BDNF Signaling Pathway (712093); Cap-dependent Translation Initiation Pathway (105967); ErbB Signaling Pathway (198844)

ncbi summary :

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

uniprot summary :

4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway. Protein type: Translation; Translation initiation. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol. Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding. Biological Process: negative regulation of translational initiation; cellular protein metabolic process; TOR signaling pathway; response to ethanol; translation; insulin receptor signaling pathway; translational initiation; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage