Histone H3 (Ab-3) Antibody | MyBioSource MBS852662 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Histone H3 (Ab-3) Antibody

catalog :

MBS852662

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS852662

products type :

Antibody

products full name :

Histone H3 (Ab-3) Antibody

products short name :

Histone H3

products name syn :

H3/a; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31; HIST1H3A; histone H3.1

other names :

histone H3.1; Histone H3.1; histone H3.1; histone H3/a; histone 1, H3a; H3 histone family, member A; histone cluster 1, H3a; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l

other gene names :

HIST1H3A; HIST1H3A; H3/A; H3FA; H3FA

uniprot entry name :

H31_HUMAN

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

136

specificity :

Histone H3 (Ab-3) antibody detects endogenous levels of total Histone H3 protein.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

app notes :

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:5000

other info1 :

Reactivity Note: Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%); Rat (Identities = 100%, Positives = 100%)

other info2 :

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H3 around the phosphorylation site of threonine 3 (A-R-TP-K-Q). Pathway: Transcription

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Zhong R., Nucleic Acids Res. 11:7409-7425(1983). Marashi F., Biochem. Cell Biol. 64:277-289(1986). Albig W., Genomics 10:940-948(1991).

ncbi gi num :

4504281

ncbi acc num :

NP_003520.1

ncbi gb acc num :

NM_003529.2

uniprot acc num :

P68431

ncbi mol weight :

15,404 Da

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amyloids Pathway (366238); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); Chromatin Modifying Enzymes Pathway (941403); Chromatin Organization Pathway (941402); Condensation Of Prophase Chromosomes Pathway (941401)

ncbi summary :

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

uniprot summary :

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 6p22.2. Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus. Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity. Biological Process: chromatin silencing at rDNA; nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; blood coagulation; DNA methylation on cytosine; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage