HP1 alpha (Phospho-Ser92) Antibody | MyBioSource MBS852359 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HP1 alpha (Phospho-Ser92) Antibody

catalog :

MBS852359

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS852359

products type :

Antibody

products full name :

HP1 alpha (Phospho-Ser92) Antibody

products short name :

HP1 alpha

products name syn :

HP1a (Phospho-Ser92) Antibody; antigen p25; chromobox protein homolog 5; heterochromatin protein 1 alpha; HP1 alpha; HP1A

other names :

chromobox protein homolog 5; Chromobox protein homolog 5; chromobox protein homolog 5; HP1-ALPHA; HP1Hs alpha; antigen p25; HP1 alpha homolog; epididymis luminal protein 25; heterochromatin protein 1-alpha; heterochromatin protein 1 homolog alpha; chromobox homolog 5 (HP1 alpha homolog, Drosophila); chromobox homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha

products gene name :

HP1

other gene names :

CBX5; CBX5; HP1; HP1A; HEL25; HP1A; HP1 alpha

uniprot entry name :

CBX5_HUMAN

host :

Rabbit

reactivity :

Human

sequence length :

191

specificity :

HP1alpha (Phospho-Ser92) antibody detects endogenous levels of HP1alpha only when phosphorylated at serine 92.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20 degree C for 1 year

tested application :

Immunohistochemistry (IHC), ELISA (EIA)

app notes :

IHC: 1:50~1:100, ELISA: 1:40000

other info1 :

Reactivity Note: Human (Identities = 100%, Positives = 100%)

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human HP1alpha around the phosphorylation site of serine 92 (R-K-SP-N-F). All Sites: Human: Ser92. Pathway: Phospho-specific

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Saunders W.S., J. Cell Sci. 104:573-582(1993). Ye Q., J. Biol. Chem. 271:14653-14656(1996). Ye Q., J. Biol. Chem. 272:14983-14989(1997).

ncbi gi num :

188035908

ncbi acc num :

NP_001120793.1

ncbi gb acc num :

NM_001127321.1

uniprot acc num :

P45973

ncbi mol weight :

22,225 Da

ncbi pathways :

Aurora B Signaling Pathway (138080); E2F Transcription Factor Network Pathway (137934); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196); Hemostasis Pathway (106028)

ncbi summary :

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

HP1 alpha: Component of heterochromatin that recognizes and binds histone H3 tails methylated at Lys-9 (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when Tyr-41 of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at Lys- 9 . Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1. Protein type: Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 12q13.13. Cellular Component: kinetochore; nucleoplasm; histone methyltransferase complex; PML body; transcriptional repressor complex; histone deacetylase complex; nucleolus; nuclear envelope; nuclear heterochromatin; nucleus; chromocenter. Molecular Function: protein binding, bridging; protein binding; protein homodimerization activity; histone deacetylase binding; chromatin binding; methylated histone residue binding. Biological Process: viral reproduction; blood coagulation; negative regulation of transcription, DNA-dependent

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage