product summary
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company name :
MyBioSource
product type :
antibody
product name :
DNA pol theta Polyclonal Antibody
catalog :
MBS8522357
quantity :
0.1 mg
price :
280 USD
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
human
application :
ELISA, immunohistochemistry
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS8522357
products type :
Antibody
products full name :
DNA pol theta Polyclonal Antibody
products short name :
[DNA pol theta]
products name syn :
[POLQ; POLH; DNA polymerase theta; DNA polymerase eta]
other names :
[DNA polymerase theta; DNA polymerase theta; DNA polymerase theta; DNA polymerase theta; DNA polymerase eta]
other gene names :
[POLQ; POLQ; PRO0327]
clonality :
Polyclonal
host :
Rabbit
reactivity :
Human
sequence length :
2590
specificity :
DNA pol Polyclonal Antibody detects endogenous levels of DNA pol protein.
purity :
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
form :
Liquid in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide.
concentration :
1 mg/ml
storage stability :
Store at -20°C/1 year.
tested application :
Immunohistochemistry (IHC), ELISA
app notes :
IHC: 1/100-1/300, ELISA: 1/20000 - Not yet tested in other applications.
other info1 :
Immunogen: Synthesized peptide derived from N-terminal region of human DNA pol at AA range: 150-230
ncbi gi num :
139394648
ncbi acc num :
NP_955452.3
ncbi gb acc num :
NM_199420.3
uniprot acc num :
O75417
ncbi mol weight :
197.597
uniprot summary :
DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed:25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed:18503084, PubMed:21050863, PubMed:22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed:14576298, PubMed:18503084, PubMed:19188258, PubMed:24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs ().
size1 :
0.1 mg
price1 :
280 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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