antibody

VEGFR2 (Phospho-Tyr1214) Antibody

MBS852192

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

VEGFR2 (Phospho-Tyr1214) Antibody

VEGFR2

EC 2.7.10.1; FLK1; KDR; kinase insert domain receptor; protein-tyrosine kinase receptor Flk-1; vascular endothelial growth factor receptor 2; VGFR2; VGR2

vascular endothelial growth factor receptor 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor 2; soluble VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; KDR; Protein-tyrosine kinase receptor flk-1; CD_antigen: CD309

VEGFR2

KDR; KDR; FLK1; CD309; VEGFR; VEGFR2; FLK1; VEGFR2; VEGFR-2; FLK-1; KDR

VGFR2_HUMAN

Rabbit

Human, Mouse, Rat

1356

VEGFR2 (Phospho-Tyr1214) antibody detects endogenous levels of VEGFR2 only when phosphorylated at tyrosine 1214.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:10000

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human VEGFR2 around the phosphorylation site of tyrosine 1214 (F-H-YP-D-N). All Sites: Human: Tyr1214; Mouse: Tyr1212; Rat: Tyr1210. Pathway: Phospho-specific

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Yin L.Y., Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases. Yu Y., Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases. Terman B.I., Oncogene 6:1677-1683(1991).

11321597

NP_002244.1

NM_002253.2

P35968

79,634 Da

Angiogenesis Pathway (198772); Axon Guidance Pathway (105688); Cardiac Progenitor Differentiation Pathway (712094); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1016174); EPHA-mediated Growth Cone Collapse Pathway (1016175); Endocytosis Pathway (102279); Endocytosis Pathway (102181)

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability. Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; VEGFR family. Chromosomal Location of Human Ortholog: 4q11-q12. Cellular Component: Golgi apparatus; endoplasmic reticulum; integral to plasma membrane; cytoplasmic membrane-bound vesicle; early endosome; plasma membrane; extracellular region; cell junction; nucleus; endosome; lipid raft; external side of plasma membrane. Molecular Function: integrin binding; vascular endothelial growth factor receptor activity; protein binding; growth factor binding; protein-tyrosine kinase activity; Hsp90 protein binding; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding. Biological Process: extracellular matrix organization and biogenesis; positive regulation of positive chemotaxis; peptidyl-tyrosine phosphorylation; viral reproduction; protein amino acid autophosphorylation; cell maturation; calcium ion homeostasis; regulation of cell shape; ovarian follicle development; positive regulation of focal adhesion formation; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; angiogenesis; vasculogenesis; endothelial cell differentiation; cell fate commitment; embryonic hemopoiesis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; cell migration during sprouting angiogenesis; branching morphogenesis of a tube; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; lymph vessel development; vascular endothelial growth factor receptor signaling pathway; surfactant homeostasis; alveolus development; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of cell migration. Disease: Hemangioma, Capillary Infantile

0.1 mg

215 USD