antibody

ERbeta (phospho Ser105) Polyclonal Antibody

MBS8520327

0.1 mg

280 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunocytochemistry, enzyme immunoassay

Antibody

ERbeta (phospho Ser105) Polyclonal Antibody

[ERbeta]

[ERS2; ESTRB; NR3A2; Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2]

[estrogen receptor beta isoform 2; Estrogen receptor beta; estrogen receptor beta; estrogen receptor 2; Nuclear receptor subfamily 3 group A member 2]

[ERbeta]

[ESR2; ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA; ESTRB; NR3A2; ER-beta]

Polyclonal

Rabbit

Human, Mouse, Rat.

495

Phospho-ERb(S105)Polyclonal Antibody detects endogenous level of ERb protein only when phosphorylated at S105.

The antibody was affinity purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

1 mg/ml

Store at -20°C/1 year.

Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)

Western Blot (WB) 1/500-1/2000. Immunofluorescence (IF) 1/200-1/1000. ELISA (EIA)1/5000. Not yet tested in other applications.

Western Blot analysis of HepG2 cells using Phospho-ERb (S105) Polyclonal antibody.

Immunogen: Synthesized peptide derived from human ERbeta around the phosphorylation site of S105

94538325

NP_001035365.1

NM_001040275.1

Q92731

59.216

Estrogen Signaling Pathway (799177); Estrogen Signaling Pathway (799197); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Integrated Pancreatic Cancer Pathway (711360); Nuclear Receptor Transcription Pathway (105979); Nuclear Receptors Pathway (198848); Ovarian Infertility Genes Pathway (198801); Plasma Membrane Estrogen Receptor Signaling Pathway (138009); Prolactin Signaling Pathway (814182)

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

0.1 mg

280 USD