antibody

Caspase 1 (Cleaved-Asp210) Antibody

MBS851976

0.1 mg

215 USD

polyclonal

rabbit

nonconjugated

ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Caspase 1 (Cleaved-Asp210) Antibody

Caspase 1

CASP-1; ICE; IL-1 beta converting enzyme; IL-1BC; IL1BCE; caspase-1; interleukin-1 beta convertase; interleukin-1 beta converting enzyme; p45

caspase-1 isoform beta; Caspase-1; caspase-1; IL1B-convertase; CASP1 nirs variant 1; IL-1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); caspase 1, apoptosis-related cysteine peptidase; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45Cleaved into the following 2 chains:Caspase-1 subunit p20; Caspase-1 subunit p10

CASP1; CASP1; ICE; P45; IL1BC; IL1BC; IL1BCE; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme

CASP1_HUMAN

Rabbit

Human

383

Caspase 1 (Cleaved-Asp210) antibody detects endogenous levels of fragment of activated Caspase 1 resulting from cleavage adjacent to Asp210.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

100ug/100ul

Store at -20 degree C for 1 year

Immunohistochemistry (IHC), ELISA (EIA)

IHC: 1:50~1:100, ELISA: 1:20000

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 1(MTTELEAFC). All Sites: Human: Asp210. Pathway: Cleaved-specific

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Victor Elharrrar, FASEB J, Mar 2006; 20: A228. R Matsumura, Ann Rheum Dis, May 2001; 60: 473 - 482. Adriana L³pez Ponte Stem Cells, Mar 2007; 10.1634/stemcells.2007-0054. A I Catrina, Ann Rheum Dis, Oct 2002; 61: 934 - 936.

4502573

NP_001214.1

NM_001223.4

P29466

10,417 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Caspase Cascade In Apoptosis Pathway (137974); Cellular Roles Of Anthrax Toxin Pathway (138076); Cytokine Signaling In Immune System Pathway (366171); Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Direct P53 Effectors Pathway (137939)

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; EC 3.4.22.36; Protease. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: cytoplasm; extracellular region; cytosol. Molecular Function: protein binding; cysteine-type endopeptidase activity; endopeptidase activity; caspase activator activity. Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; membrane hyperpolarization; interleukin-1 beta production; response to lipopolysaccharide; positive regulation of interleukin-1 beta secretion; mitochondrial depolarization; proteolysis; signal transduction; response to ATP; positive regulation of interleukin-1 alpha secretion; regulation of inflammatory response; response to hypoxia; innate immune response; regulation of autophagy

0.1 mg

215 USD