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Merlin (Phospho-Ser10) Antibody

MBS851974

0.1 mg

280 USD

Antibody

Merlin (Phospho-Ser10) Antibody

[Merlin]

[moesin-ezrin-radizin-like protein; neurofibromatosis type 2 gene; neurofibromatosis-2; Neurofibromin 2; NF-2; NF2; SCH; Schwannomerlin; schwannomerlin; Schwannomin]

[merlin isoform 1; Merlin; merlin; neurofibromin 2; Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin]

[MERL]

[NF2; NF2; ACN; SCH; BANF; SCH]

Human, Mouse, Rat

595

Merlin (Phospho-Ser10) antibody detects endogenous levels of Merlin only when phosphorylated at serine 10.

Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at-20 degree C for 1 year.

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

WB: 1:500~1:1000. IHC: 1:50~1:100. ELISA: 1:1000

Testing Data

Immunohistochemistry (IHC)

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Merlin around the phosphorylation site of serine 10 (R-M-SP-F-S).

All Sites: Human: Ser10. Mouse: Ser10. Rat: Ser6

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

Trofatter J.A., Cell 72:791-800(1993). Rouleau G.A., Nature 363:515-521(1993). Zucman-Rossi J., Hum. Mol. Genet. 7:2095-2101(1998).

4557795

NP_000259.1

NM_000268.3

P35240

24,515 Da

ErbB2/ErbB3 Signaling Events Pathway (137955); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.

0.1 mg

280 USD