Zyxin (Phospho-Ser142) Antibody | MyBioSource MBS851664 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Zyxin (Phospho-Ser142) Antibody

catalog :

MBS851664

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS851664

products type :

Antibody

products full name :

Zyxin (Phospho-Ser142) Antibody

products short name :

Zyxin

products name syn :

ZYX; Zyxin 2

other names :

zyxin; Zyxin; zyxin; zyxin-2; zyxin; Zyxin-2

other gene names :

ZYX; ZYX; ESP-2; HED-2

uniprot entry name :

ZYX_HUMAN

host :

Rabbit

reactivity :

Human

sequence length :

572

specificity :

Zyxin (Phospho-Ser142) antibody detects endogenous levels of Zyxin only when phosphorylated at serine 142.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

WB: 1:500~1:1000, ELISA: 1:1000

other info1 :

Reactivity Note: Human (Identities = 100%, Positives = 100%)

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Zyxin around the phosphorylation site of serine 142 (K-V-SP-S-I). All Sites: Human: Ser142. Pathway: Phospho-specific

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Macalma T., J. Biol. Chem. 271:31470-31478(1996). Zumbrunn J., Eur. J. Biochem. 241:657-663(1996). Hillier L.W., Nature 424:157-164(2003). ----------------------------------------------------------------------------

ncbi gi num :

58530845

ncbi acc num :

NP_001010972.1

ncbi gb acc num :

NM_001010972.1

uniprot acc num :

Q15942

ncbi mol weight :

61,277 Da

ncbi pathways :

E-cadherin Signaling In Keratinocytes Pathway (138002); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Integrin-mediated Cell Adhesion Pathway (198816); Stabilization And Expansion Of The E-cadherin Adherens Junction Pathway (137918)

ncbi summary :

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]

uniprot summary :

Zyxin: Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression. Interacts with HPV type 6 protein E6. Does not interact significantly with E6 proteins from HPV types 11, 16, or 18. Interacts, via the Pro-rich regions, with the EVH1 domains of ENAH, EVL and VASP. Interacts with the first LIM domain of TES. Interacts with NEBL (isoform 2). Belongs to the zyxin/ajuba family. Protein type: Motility/polarity/chemotaxis; Cell adhesion. Chromosomal Location of Human Ortholog: 7q32. Cellular Component: cell-cell adherens junction; focal adhesion; integral to plasma membrane; cytoplasm; stress fiber; plasma membrane; nucleus. Molecular Function: protein binding; zinc ion binding. Biological Process: integrin-mediated signaling pathway; cell-cell signaling; viral reproduction; cell-matrix adhesion; transforming growth factor beta receptor signaling pathway; regulation of inflammatory response; stress fiber formation; cell adhesion; signal transduction

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage