antibody

SIRT1 Antibody

MBS850730

0.1 mg

290 USD

monoclonal

mouse

nonconjugated

1F3

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

Antibody

SIRT1 Antibody

SIRT1

SIR2L1; SIRT1

NAD-dependent protein deacetylase sirtuin-1 isoform b; NAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1; sirtuin 1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2

SIRT1

SIRT1; SIRT1; SIR2L1; SIR2L1; hSIRT1; hSIR2; 75SirT1

SIR1_HUMAN

Monoclonal

1F3

Mouse

Human, Monkey

452

Ascitic fluid containing 0.03% sodium azide.

100ug/100ul

Store at 4 degree C, for long term storage, store at -20 degree C.

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

WB: 1/500 - 1/2000, IHC: 1/200-1/1000, IF: 1/200-1/1000, FC: 1/200-1/400, ELISA: Propose dilution 1/10000. Not yet tested in other applications.

Immunogen: Purified recombinant fragment of human SIRT1 expressed in E. Coli. Species: Mouse IgG1

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Background: The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta.

215982798

NP_001135970.1

NM_001142498.1

120kDa

AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!Amphetamine Addiction Pathway 547607!!Amphetamine Addiction Pathway 550546!!Androgen Receptor Signaling Pathway 198806!!Cellular Response To Heat Stress Pathway 980470!!Cellular Responses To Stress Pathway 645258!!E2F Transcription Factor Network Pathway 137934!!Energy Metabolism Pathway 198907!!Epigenetic Regulation Of Gene Expression Pathway 980449

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

0.1 mg

290 USD