ELISA/assay

Human IL-1beta ELISA Kit

MBS8506075

1 x48 Wells

295 USD

ELISA Kit

Human IL-1beta ELISA Kit

IL-1beta

interleukin 1, beta; interleukin 1, beta; interleukin-1 beta

IL1B2; IL1B; IL-1beta

267

This assay recognizes both natural and recombinant human IL-1beta. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.

Samples: Cell culture supernates, serum, and plasma. Assay Type: Sandwich. Sensitivity: 2pg/mL.

Immunology

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1beta present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1beta is added to the wells and binds to the combination of capture antibody-IL-1beta in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of IL-1beta present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1beta standard dilutions and IL-1beta sample concentration determined. Background: Interleukin-1 (IL-1), originally described as lymphocyte activating factor (LAF) for its effects on thymocytes(1), is a polypeptide cytokine with two molecular forms. The two distinct molecular forms of IL-1 are thought to be derived from two genes(2,3). After transcription, as 31kD precursor polypeptide is cleaved to give rise to mostly cell membrane associated IL-1alpha and secreted IL-1beta. Both have the same molecular weight of 15kD but have different isoelectric points of 5 and 7, respectively. Despite sequence homology of only 20%, both forms are thought to bind to the same receptor(4). IL-1 inhibitors that vary only in their degree of glycosylation have been described to bind to the IL-1 receptor(5). These inhibitors are structurally related to IL-1beta and may be important in regulation of IL-1beta action(6). IL-1beta is produced primarily by monocytes and macrophages (7) but also by astrocytes, oligodendroglia, adrenal cortical cells, NK cells, endothelial cells, keratinocytes, megakaryocytes, platelets, neurons, neutrophils, osteoblasts, Schwann cells, trophoblasts, T cells, and fibroblasts. IL-1 has multiple immunological functions including enhancement of IL-2 production by T cells and activation of B-cells (BAF) and thymocytes(8-10). A true pleiotrope, IL-1 may have tumoricidal activity via its release of IL-2 and Interferon gamma and indirectly antiviral by stimulating to release interferon beta(11). Low levels of IL-1beta have been reported in normal serum(12). It is thought that IL-1 genes are induced to respond to tissue damage or in infection. Elevated levels have been reported in a number of infectious disease conditions and in noninfectious inflammatory conditions such as Crohn's disease. In addition to elevated serum levels, IL-1 has been found in synovial fluids of patients with rheumatoid arthritis and in cerebrospinal fluid after neurological inflammation or insult(13,14). At the other end of the spectrum, low levels of IL-1 have been found in malnutrition and advanced neoplasia suggesting perhaps a complex immunological and physiological regulatory role for this cytokine.

697663067

NP_001289317.1

NM_001302388.1

African Trypanosomiasis Pathway (194379); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (84508); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167313); Amoebiasis Pathway (167191); Apoptosis Pathway (84472); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795)

1 x48 Wells

295 USD

1x96 Wells

355