RAD51 (Phospho-Thr309) Antibody | MyBioSource MBS8505653 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

RAD51 (Phospho-Thr309) Antibody

catalog :

MBS8505653

quantity :

0.1 mg

price :

215 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS8505653

products type :

Antibody

products full name :

RAD51 (Phospho-Thr309) Antibody

products short name :

RAD51

products name syn :

DNA repair protein RAD51 homolog 1; RAD51A; RECA

other names :

DNA repair protein RAD51 homolog 1 isoform 2; DNA repair protein RAD51 homolog 1; DNA repair protein RAD51 homolog 1; RAD51 homolog A; RecA-like protein; recombination protein A; RecA, E. coli, homolog of; BRCA1/BRCA2-containing complex, subunit 5; RAD51 recombinase; RAD51 homolog A

products gene name :

RAD51

other gene names :

RAD51; RAD51; RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930; RAD51A; RECA; HsRAD51; hRAD51

uniprot entry name :

RAD51_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

340

specificity :

RAD51 (Phospho-Thr309) antibody detects endogenous levels of RAD51 only when phosphorylated at threonina 309.

purity :

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography useing non-phospopeptid corresponding to the phosphorylation s

form :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

concentration :

1mg/ml

storage stability :

Store at -20 degree C for 1 year

tested application :

Western Blot (WB), ELISA (EIA)

app notes :

IHC: 1:50~1:100, ELISA: 1:1000

other info2 :

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human RAD51 around the phosphorylation site of threonina 309(G-E-TP-R-I). All Sites: Human: Thr309; Mouse: Thr309

products categories :

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

products references :

Shinohara A., Nat. Genet. 4:239-243(1993). Yoshimura Y., Nucleic Acids Res. 21:1665-1665(1993). Schmutte C., Cancer Res. 59:4564-4569(1999).

ncbi gi num :

256017143

ncbi acc num :

NP_001157741.1

ncbi gb acc num :

NM_001164269.1

uniprot acc num :

Q06609

ncbi pathways :

Assembly Of The RAD51-ssDNA Nucleoprotein Complex Pathway (105873); BARD1 Signaling Events Pathway (137959); Cell Cycle Pathway (530733); DNA Repair Pathway (105837); DNA Damage Response Pathway (198788); Double-Strand Break Repair Pathway (105861); Fanconi Anemia Pathway (377262); Fanconi Anemia Pathway (377128); Homologous DNA Pairing And Strand Exchange Pathway (105870); Homologous Recombination Repair Pathway (105862)

ncbi summary :

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

uniprot summary :

RAD51: a member of the RAD51 protein family. Involved in the homologous recombination and repair of DNA. Interacts with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. Interacts with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both its intracellular localization and DNA-binding ability. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Defects in RAD51 are associated with breast cancer. Two alternatively spliced isoforms have been reported. Protein type: Mitochondrial; DNA repair, damage. Chromosomal Location of Human Ortholog: 15q15.1. Cellular Component: nuclear chromosome; PML body; condensed nuclear chromosome; mitochondrion; condensed chromosome; nucleoplasm; lateral element; mitochondrial matrix; perinuclear region of cytoplasm; cytoplasm; nucleolus; microtubule organizing center; nucleus. Molecular Function: protein C-terminus binding; identical protein binding; protein binding; double-stranded DNA binding; damaged DNA binding; single-stranded DNA-dependent ATPase activity; ATP binding; single-stranded DNA binding. Biological Process: DNA unwinding during replication; meiosis; DNA recombinase assembly; mitotic recombination; meiotic recombination; double-strand break repair; positive regulation of DNA ligation; DNA repair; response to DNA damage stimulus; protein homooligomerization; double-strand break repair via homologous recombination; DNA recombination. Disease: Breast Cancer; Mirror Movements 2

size1 :

0.1 mg

price1 :

215 USD

more info or order :

MyBioSource product webpage