antibody

Mouse anti-Human CD56, FITC Conjugated mAb

MBS8504188

50 Tests

215 USD

monoclonal

mouse

FITC

[4AMEM188]

immunocytochemistry, flow cytometry, FACS

Antibody

Mouse anti-Human CD56, FITC Conjugated mAb

[CD56]

[CD56; CD56; CD56]

[CD56]

[CD56]

Monoclonal

IgG2a

[4AMEM188]

Mouse

Human

848

Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide

Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Testing

Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube; . 2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light; . 3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×); . 4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant; . 5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant; . 6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured). [PBS wash buffer: PBS +1% FBS +0.1% NaN3]. [Cell fixation: 2% formaldehyde solution]

4AMEM188 reacts with CD56, a 175-220 kDa Neural Cell Adhesion Molecule (NCAM), expressed on 10-25% of peripheral blood lymphocytes, including all CD16+ NK cells and approximately 5% of CD3+ lymphocytes, referred to as NKT cells. It also is present at brain and neuromuscular junctions, certain LGL leukemias, small cell lung carcinomas, neuronally derived tumors, myeloma and myeloid leukemias. CD56 (NCAM) is involved in neuronal homotypic cell adhesion which is implicated in neural development, and in cell differentiation during embryogenesis.

1. Knapp, W et al., eds. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York. 2. Lanier LL. et al. (1991) J. Immunol. 146:4421-4426. 3. Campbell, JJ et al. (2001) J. Immunol. 166:6477-6482. 4. Cooper MD et al. (2001) Trends in Immunol. 22:633-640. 5. Galandrini R et al. (2002) Blood. 100:4581-4589.

158524860

ABW71276.1

93,063 Da

Axon Guidance Pathway (105688); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine Signaling In Immune System Pathway (366171); Developmental Biology Pathway (477129); FGF Signaling Pathway (137989); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Gamma Signaling Pathway (187106); L1CAM Interactions Pathway (161003)

50 Tests

215 USD

100 Tests

280