catalog number :
MBS850209
products full name :
Flag-Tag monoclonal antibody
products short name :
Flag-Tag
reactivity :
Human, Mouse, Rat
specificity :
Recognizes over-expressed proteins containing Flag epitope tag (D-tag) fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
form :
Mouse Monoclonal (IgG2b). 1mg/ml in PBS (pH 7.2) with 50% glycerol. Frozen liquid.
concentration :
50ug/50ul
storage stability :
Stable for one year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4 degree C for 3 months.
tested application :
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
app notes :
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows: Western Blot at dilutions of 1:1000-4000. Immunoprecipitation and immunostaining at dilutions of 1:200-400.
other info2 :
Immunogen: A synthetic peptide (DYKDDDDK) coupled to KLH. Pathway: other product
products categories :
Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
products references :
Webster NL, et al. (2005) Immunol Cell Biol; 83(5): 542-548.